Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nagai, M.; Dave, V.; Kaplan, B.E.; Yoshida, A.
    Human blood group glycosyltransferases. I. Purification of N-acetylgalactosaminyltransferase (1987), J. Biol. Chem., 253, 377-379.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.40 enzyme is not stable in crude plasma Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.40 Mn2+ 15-30 mM required for maximal activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.40 52000
-
 2 * 52000, SDS-PAGE of carboxymaleyl enzyme Homo sapiens
2.4.1.40 90000 100000 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.40 Homo sapiens
-
 blood group A1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.40
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.40 blood plasma blood group A1 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.40 additional information
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.4.1.40 -60°C, 2 mM MnCl2, 1 mM EDTA, 0.02 mM UDP, stable for up to 30 days Homo sapiens
2.4.1.40 4°C, 25% loss of activity after storage of plasma for 2 weeks Homo sapiens
2.4.1.40 4°C, rapid loss of activity of purified enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.40 UDP-N-acetyl-D-galactosamine + 2'-fucosyllactose
-
 Homo sapiens UDP + N-acetyl-alpha-D-galactosaminyl-2'-fucosyllactose
-
 ?
2.4.1.40 UDP-N-acetyl-D-galactosamine + glycoprotein alpha-L-fucosyl-1,2-D-galactose
-
 Homo sapiens UDP + glycoprotein N-acetyl-alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.40 dimer 2 * 52000, SDS-PAGE of carboxymaleyl enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.40 6.5 7
-
 Homo sapiens