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Literature summary extracted from

  • MacKenzie, C.R.; Johnson, K.G.; McDonald, I.J.
    Glycogen synthesis by amylosucrase from Neisseria perflava (1977), Can. J. Microbiol., 23, 1303-1307.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.4 D-fructose competitive inhibition Neisseria perflava
2.4.1.4 sucrose above 100 mM, substrate inhibition Neisseria perflava
2.4.1.4 Tris-HCl buffer
-
 Neisseria perflava

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.4 additional information in cells grown with sucrose, probably bound to polymerized product Neisseria perflava
-
-
2.4.1.4 soluble in cells grown without sucrose Neisseria perflava
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.4 sucrose + (1,4-alpha-D-glucosyl)n Neisseria perflava constitutive enzyme D-fructose + (1,4-alpha-D-glucosyl)n+1
-
 ?
2.4.1.4 sucrose + (1,4-alpha-D-glucosyl)n Neisseria perflava NRC 31001 constitutive enzyme D-fructose + (1,4-alpha-D-glucosyl)n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.4 Neisseria perflava
-
-
-
2.4.1.4 Neisseria perflava NRC 31001
-
-
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.4 -20°C, several months, no loss of activity Neisseria perflava

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.4 sucrose + (1,4-alpha-D-glucosyl)n transfers glucose to growing alpha-1,4-glucan chains Neisseria perflava D-fructose + (1,4-alpha-D-glucosyl)n+1
-
 ?
2.4.1.4 sucrose + (1,4-alpha-D-glucosyl)n constitutive enzyme Neisseria perflava D-fructose + (1,4-alpha-D-glucosyl)n+1
-
 ?
2.4.1.4 sucrose + (1,4-alpha-D-glucosyl)n transfers glucose to growing alpha-1,4-glucan chains Neisseria perflava NRC 31001 D-fructose + (1,4-alpha-D-glucosyl)n+1
-
 ?
2.4.1.4 sucrose + (1,4-alpha-D-glucosyl)n constitutive enzyme Neisseria perflava NRC 31001 D-fructose + (1,4-alpha-D-glucosyl)n+1
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.4 6.7 7
-
 Neisseria perflava

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.4 20
-
 D-fructose
-
 Neisseria perflava