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Literature summary extracted from

  • Carne, L.R.; Watkins, W.M.
    Human blood group B gene-specified alpha-3-galactosyltransferase: purification of the enzyme in serum by biospecific adsorption onto blood group O erythrocyte membranes (1977), Biochem. Biophys. Res. Commun., 77, 700-707.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.37 UDP competitive with respect to UDP-galactose Homo sapiens
2.4.1.37 UDP-N-acetylgalactosamine weak, competitive with respect to UDP-galactose Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.37 0.01
-
 UDP-galactose
-
 Homo sapiens
2.4.1.37 0.5
-
 2'-fucosyllactose
-
 Homo sapiens
2.4.1.37 2.2
-
 O-alpha-L-fucosyl-1,2-galactose
-
 Homo sapiens
2.4.1.37 2.5
-
 lacto-N-fucopentaose I
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.37 Co2+ slight activation Homo sapiens
2.4.1.37 Mn2+ divalent cation required, Mn2+ most effective, optimum concentration 20 mM Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.37 Homo sapiens
-
 blood group B donors
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.37 one-step procedure involving absorption onto group 0 erythrocyte membranes followed by elution with the low molecular weight H-active trisaccharide 2'-fucosyllactose Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.37 serum
-
 Homo sapiens
-
2.4.1.37 stomach
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.37 1.92
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.37 UDP-galactose + 2'-fucosyllactose
-
 Homo sapiens UDP + alpha-D-galactosyl-1,3-[2'-fucosyllactose]
-
 ?
2.4.1.37 UDP-galactose + glycoprotein alpha-L-fucosyl-1,2-D-galactose enzyme transfers D-galactose in alpha-linkage to oligosaccharides, glycolipids and glycoproteins with terminal non-reducing H-active structures and confers blood group B activity on group 0 erythrocytes Homo sapiens UDP + glycoprotein alpha-D-galactosyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose
-
 ?
2.4.1.37 UDP-galactose + H-active glycoprotein
-
 Homo sapiens UDP + B-active substance
-
 ?
2.4.1.37 UDP-galactose + lacto-N-fucopentaose I
-
 Homo sapiens UDP + alpha-D-galactosyl-1,3-[lacto-N-fucopentaose I]
-
 ?
2.4.1.37 UDP-galactose + O-alpha-L-fucosyl-1,2-galactose
-
 Homo sapiens UDP + alpha-D-galactosyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-galactopyranoside
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.37 55
-
 5 min, 20% loss of activity, 20 min, 60% loss of activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.37 6.5
-
-
 Homo sapiens