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Literature summary extracted from

  • Seto, N.O.L.; Palcic, M.M.; Compston, C.A.; Li, H.; Bundle, D.R.; Narang, S.A.
    Sequential interchange of four amino acids from blood group B to blood group A glycosyltransferase boosts catalytic activity and progressively modifies substrate recognition in human recombinant enzymes (1997), J. Biol. Chem., 272, 14133-14138.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.37 expressed in Escherichia coli TG-1 Homo sapiens
2.4.1.40 expressed in Escherichia coli TG-1 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.37 0.034
-
 UDP-galactose
-
 Homo sapiens
2.4.1.37 0.054
-
 L-fucosyl-alpha-1,2-beta-D-galactosyl-O(CH2)7CH3 with UDP-galactose as donor Homo sapiens
2.4.1.37 0.281
-
 L-fucosyl-alpha-1,2-beta-D-galactosyl-O(CH2)7CH3 with UDP-N-acetylgalactosamine as donor Homo sapiens
2.4.1.37 0.285
-
 UDP-N-acetylgalactosamine
-
 Homo sapiens
2.4.1.40 0.013
-
 L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
-
 Homo sapiens
2.4.1.40 0.015
-
 UDP-GalNAc
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.37 Homo sapiens
-
-
-
2.4.1.40 Homo sapiens
-
 only four amino acids are responsible for B and A blood group enzymes R176G, G235S, L266M and G268A, the latter two amino acids are responsible for the difference in donor specificity and the first two have roles in acceptor binding and turnover
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.37 UDP-N-acetylgalactosamine + L-fucosyl-alpha-1,2-beta-D-galactosyl-O(CH2)7CH3
-
 Homo sapiens UDP + N-acetyl-alpha-D-galactosyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-galactosyl-O(CH2)7CH3
-
 ?
2.4.1.40 UDP-Gal + L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
-
 Homo sapiens ?
-
 ?
2.4.1.40 UDP-N-acetyl-D-galactosamine + L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
-
 Homo sapiens UDP + alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-galactosyl-O(CH2)7CH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.37 GTB
-
 Homo sapiens
2.4.1.40 GTA
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.37 0.005
-
 UDP-N-acetylgalactosamine
-
 Homo sapiens
2.4.1.37 0.108
-
 UDP-galactose
-
 Homo sapiens
2.4.1.40 0.000333
-
 UDP-Gal
-
 Homo sapiens
2.4.1.40 0.0817
-
 UDP-GalNAc
-
 Homo sapiens