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Literature summary extracted from

  • Marcus, S.L.; Polakowski, R.; Seto, N.O.L.; Leinala, E.; Borisova, S.; Blancher, A.; Roubinet, F.; Evans, S.V.; Palcic, M.M.
    A single point mutation reverses the donor specificity of human blood group B-synthesizing galactosyltransferase (2003), J. Biol. Chem., 278, 12403-12405.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.37 P234S-mutant expressed in Escherichia coli BL21 Homo sapiens
2.4.1.40 expressed in Escherichia coli BL21, P234S-mutant of blood-group substance B-dependent galactosyltransferase Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.37 P234S-mutant, 1.55 and 1.65 A resolution, with and without H-antigen Homo sapiens
2.4.1.40 G176R/P234S/S235G/M266L/A268G-mutant with and without H-antigen, at 1.55 and 1.65 A resolution respectively Homo sapiens
2.4.1.40 of catalytic domain residues 63-354, with and without L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3-acceptor and UDP, at 1.35 and 1.8 A resolution respectively Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.37 P234S dramatic and complete reversal of donor specificity, it preferentially utilizes UDP-GalNac for transfer Homo sapiens
2.4.1.40 G176R/P234S/S235G/M266L/A268G expressed in Escherichia coli BL21, although 4 of the mutations correspond to a change from A- to B-blood group, the P234S-mutation shows a remarkable increase in A-donor specificity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.37 0.027
-
 L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
-
 Homo sapiens
2.4.1.37 0.088
-
 UDP-galactose
-
 Homo sapiens
2.4.1.37 0.106
-
 L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3 P234S mutant Homo sapiens
2.4.1.37 4.5
-
 UDP-galactose P234S mutant Homo sapiens
2.4.1.40 0.0087
-
 L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
-
 Homo sapiens
2.4.1.40 0.0099
-
 UDP-GalNAc
-
 Homo sapiens
2.4.1.40 0.144
-
 L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
-
 Homo sapiens
2.4.1.40 0.167
-
 L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3 G176R/P234S/S235G/M266L/A268G mutant Homo sapiens
2.4.1.40 0.242
-
 UDP-GlcNAc
-
 Homo sapiens
2.4.1.40 3.74
-
 UDP-GalNAc G176R/P234S/S235G/M266L/A268G mutant Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.37 Mn2+
-
 Homo sapiens
2.4.1.40 Mn2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.40 additional information Homo sapiens transfers N-acetylgalactosamine from UDP-N-acetylgalactosamine to H-active structures to form A determinants ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.37 Homo sapiens P16442 only four amino acids are responsible for B and A blood group enzymes R176G, G235S, L266M and G268A, the latter two amino acids are responsible for the difference in donor specificity and the first two have roles in acceptor binding and turnover
-
2.4.1.40 Homo sapiens P16442 only four amino acids are responsible for B and A blood group enzymes R176G, G235S, L266M and G268A, the latter two amino acids are responsible for the difference in donor specificity and the first two have roles in acceptor binding and turnover
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.37 UDP-galactose + glycoprotein alpha-L-fucosyl-1,2-D-galactose
-
 Homo sapiens UDP + glycoprotein alpha-D-galactosyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose
-
 ?
2.4.1.37 UDP-galactose + L-fucosyl-alpha-1,2-beta-D-galactosyl-O(CH2)7CH3
-
 Homo sapiens UDP + alpha-D-galactosyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-galactosyl-O(CH2)7CH3
-
 ?
2.4.1.40 additional information transfers N-acetylgalactosamine from UDP-N-acetylgalactosamine to H-active structures to form A determinants Homo sapiens ?
-
 ?
2.4.1.40 UDP-N-acetyl-D-galactosamine + L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
-
 Homo sapiens UDP + alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-galactosyl-O(CH2)7CH3
-
 ?
2.4.1.40 UDP-N-acetyl-D-glucosamine + L-fucosyl-alpha-1,2-beta-glucosyl-O(CH2)7CH3
-
 Homo sapiens UDP + N-acetyl-alpha-D-glucosaminyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-glucosyl-O(CH2)7CH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.37 GTB
-
 Homo sapiens
2.4.1.40 GTA
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.37 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.37 0.004
-
 UDP-galactose P234S mutant Homo sapiens
2.4.1.37 0.085
-
 UDP-galactose
-
 Homo sapiens
2.4.1.40 0.24
-
 UDP-GalNAc G176R/P234S/S235G/M266L/A268G mutant Homo sapiens
2.4.1.40 0.292
-
 UDP-GalNAc wild-type Homo sapiens