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Literature summary extracted from

  • Kitaoka, M.; Sasaki, T.; Taniguchi, H.
    Purification and properties of laminaribiose phosphorylase (EC 2.4 1.31) from Euglena gracilis Z (1993), Arch. Biochem. Biophys., 304, 508-514.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.31 D-glucose substrate inhibition at high concentration Euglena gracilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.31 120000
-
 2 * 120000, SDS-PAGE Euglena gracilis
2.4.1.31 200000
-
 gel filtration Euglena gracilis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.31 Euglena gracilis
-
 var. bacillaris, IAM E-2 and IAM E-3, Z. IAM E-6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.31 3 isoforms: F0, FI, FII Euglena gracilis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.31 3-beta-D-glucosyl-D-glucose + phosphate = D-glucose + alpha-D-glucose 1-phosphate ordered bi-bi mechanism Euglena gracilis
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.31 additional information
-
-
 Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.31 3-beta-D-glucosyl-D-glucose + phosphate
-
 Euglena gracilis D-glucose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
-
 Euglena gracilis ?
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + cellobiose
-
 Euglena gracilis phosphate + oligosaccharides
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + D-glucose initial rate of synthetic reaction is 4 times faster than that of phosphorolytic reaction Euglena gracilis laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + laminaribiose
-
 Euglena gracilis ?
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + m-nitrophenyl-beta-D-glucoside
-
 Euglena gracilis ?
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + methyl beta-D-glucoside
-
 Euglena gracilis ?
-
 ?
2.4.1.31 alpha-D-glucose 1-phosphate + p-nitrophenyl-beta-D-glucoside
-
 Euglena gracilis ?
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside
-
 Euglena gracilis ?
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + salicin
-
 Euglena gracilis ?
-
 r
2.4.1.31 laminaripentaose + phosphate
-
 Euglena gracilis laminaritetraose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.31 laminaritetraose + phosphate
-
 Euglena gracilis laminaritriose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.31 laminaritriose + phosphate
-
 Euglena gracilis laminaribiose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.31 additional information weak or no activity observed with other carbohydrates Euglena gracilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.31 dimer 2 * 120000, SDS-PAGE Euglena gracilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.31 30
-
 assay at Euglena gracilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.31 35
-
 isoenzyme F1, stable below Euglena gracilis
2.4.1.31 40
-
 isoenzymes F0 and F2, stable below Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.31 7.2
-
 in Tris-HCl buffer Euglena gracilis