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Literature summary extracted from

  • Manners, D.J.; Taylor, D.C.
    Studies on carbohydrate metabolizing enzymes. XVI. Specificity of laminaribiose phosphorylase from Astasia ocellata (1967), Arch. Biochem. Biophys., 121, 443-451.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.31 EDTA markedly increased activity Astasia ocellata

General Stability

EC Number General Stability Organism
2.4.1.31 aqueous extracts not stable Astasia ocellata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.31 Ca2+ slight activation Astasia ocellata

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.31 Astasia ocellata
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.31 3-beta-D-glucosyl-D-glucose + phosphate the enzyme is specific for the alpha-isomer Astasia ocellata D-glucose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + cellobiose
-
 Astasia ocellata phosphate + oligosaccharides oligosaccharides with two or more 1,3-linked glucose residues attached to the nonreducing residue of cellobiose r
2.4.1.31 alpha-D-glucose 1-phosphate + D-glucose at equilibrium synthesis of laminaribiose is favoured Astasia ocellata laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + D-glucose specific for alpha-D-glucose 1-phosphate as glucosyl donor Astasia ocellata laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + laminaribiose
-
 Astasia ocellata ?
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + laminaritriose
-
 Astasia ocellata ?
-
 r
2.4.1.31 alpha-D-glucose 1-phosphate + methyl beta-D-glucoside
-
 Astasia ocellata ?
-
 ?
2.4.1.31 alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside
-
 Astasia ocellata ?
-
 r
2.4.1.31 additional information in presence of arsenate, laminaribiose is completely converted into glucose Astasia ocellata ?
-
 ?
2.4.1.31 additional information weak or no activity observed with other carbohydrates Astasia ocellata ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.31 37
-
 assay at Astasia ocellata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.31 21
-
 5%, 9%, 24% and 37% loss of activity after 2 h, 6 h, 24 h and 48 h, respectively Astasia ocellata