EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.31 | EDTA | markedly increased activity | Astasia ocellata |
EC Number | General Stability | Organism |
---|---|---|
2.4.1.31 | aqueous extracts not stable | Astasia ocellata |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.31 | Ca2+ | slight activation | Astasia ocellata |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.31 | Astasia ocellata | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.31 | 3-beta-D-glucosyl-D-glucose + phosphate | the enzyme is specific for the alpha-isomer | Astasia ocellata | D-glucose + alpha-D-glucose 1-phosphate | - |
r | |
2.4.1.31 | alpha-D-glucose 1-phosphate + cellobiose | - |
Astasia ocellata | phosphate + oligosaccharides | oligosaccharides with two or more 1,3-linked glucose residues attached to the nonreducing residue of cellobiose | r | |
2.4.1.31 | alpha-D-glucose 1-phosphate + D-glucose | at equilibrium synthesis of laminaribiose is favoured | Astasia ocellata | laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate | - |
r | |
2.4.1.31 | alpha-D-glucose 1-phosphate + D-glucose | specific for alpha-D-glucose 1-phosphate as glucosyl donor | Astasia ocellata | laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate | - |
r | |
2.4.1.31 | alpha-D-glucose 1-phosphate + laminaribiose | - |
Astasia ocellata | ? | - |
r | |
2.4.1.31 | alpha-D-glucose 1-phosphate + laminaritriose | - |
Astasia ocellata | ? | - |
r | |
2.4.1.31 | alpha-D-glucose 1-phosphate + methyl beta-D-glucoside | - |
Astasia ocellata | ? | - |
? | |
2.4.1.31 | alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside | - |
Astasia ocellata | ? | - |
r | |
2.4.1.31 | additional information | in presence of arsenate, laminaribiose is completely converted into glucose | Astasia ocellata | ? | - |
? | |
2.4.1.31 | additional information | weak or no activity observed with other carbohydrates | Astasia ocellata | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.31 | 37 | - |
assay at | Astasia ocellata |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.31 | 21 | - |
5%, 9%, 24% and 37% loss of activity after 2 h, 6 h, 24 h and 48 h, respectively | Astasia ocellata |