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Literature summary extracted from

  • Kitaoka, M.; Hayashi, K.
    Carbohydrate-processing phosphorolytic enzymes (2002), Trends Glycosci. Glycotechnol., 14, 35-50.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.30 additional information absolute requirement for sulfhydryl groups Euglena gracilis

Application

EC Number Application Comment Organism
2.4.1.30 synthesis synthesis of laminarioligosaccharides, degree of polymerization is dependent on the glucose/glucose 1-phosphate ratio, overview Euglena gracilis
2.4.1.30 synthesis synthesis of laminarioligosaccharides by combined action of laminaribiose phosphorylase EC 2.4.1.31 and beta-1,3-oligoglucan phosphorylase EC 2.4.1.30 Euglena gracilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.30 (1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate Euglena gracilis role in utilization of the storage polysaccharide paramylon, i.e. beta-1,3-glucan (1,3-beta-D-glucosyl)n + phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.30 Euglena gracilis
-
-
-
2.4.1.30 Ochromonas malhamensis
-
-
-
2.4.1.31 Euglena gracilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.30
-
 Ochromonas malhamensis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.30 [(1->3)-beta-D-glucosyl]n + phosphate = [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate inversion mechanism Euglena gracilis
a
2.4.1.31 3-beta-D-glucosyl-D-glucose + phosphate = D-glucose + alpha-D-glucose 1-phosphate ordered bi-bi mechanism Euglena gracilis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.30 (1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 Euglena gracilis (1,3-beta-D-glucosyl)n + phosphate
-
 r
2.4.1.30 (1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
-
 Ochromonas malhamensis (1,3-beta-D-glucosyl)n + phosphate
-
 r
2.4.1.30 (1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate role in utilization of the storage polysaccharide paramylon, i.e. beta-1,3-glucan Euglena gracilis (1,3-beta-D-glucosyl)n + phosphate
-
 r
2.4.1.30 laminaribiose + phosphate
-
 Euglena gracilis D-glucose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.30 laminaritriose + phosphate
-
 Euglena gracilis laminaribiose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.30 paramylon + phosphate
-
 Ochromonas malhamensis ?
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.1.30 laminaridextrin phosphorylase
-
 Euglena gracilis
2.4.1.30 laminaridextrin phosphorylase
-
 Ochromonas malhamensis
2.4.1.30 More EC 2.4.1.30 and EC 2.4.1.31 catalyze the same reaction but with different quantitative specificity, EC 2.4.1.30 phosphorolyzes laminaritriose and higher homologues at a greater rate than laminaribiose, while the opposite behavior is observed with EC 2.4.1.31 i.e. laminaribiose phosphorylase Euglena gracilis