EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.26 | phosphate buffer | stimulates | Tequatrovirus T4 | |
2.4.1.26 | sulfhydryl reagents | required | Enterobacteria phage T6 | |
2.4.1.28 | sulfhydryl reagents | required | Enterobacteria phage T6 |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.26 | MgCl2 | - |
Enterobacteria phage T6 | |
2.4.1.26 | MgCl2 | stimulates | Tequatrovirus T4 | |
2.4.1.28 | Mg2+ | required for activity | Enterobacteria phage T6 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.28 | UDP-glucose + glucosylhydroxymethylcytosine residue in DNA | Enterobacteria phage T6 | - |
UDP + beta-D-glucosyl-beta-D-glucosyl-hydroxymethylcytosine containing DNA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.26 | Enterobacteria phage T6 | - |
Escherichia coli infected with | - |
2.4.1.26 | Escherichia phage T2 | - |
Escherichia coli infected with | - |
2.4.1.26 | Tequatrovirus T4 | - |
Escherichia coli infected with | - |
2.4.1.28 | Enterobacteria phage T6 | - |
Escherichia coli infected with | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.26 | partial, E. coli infected with | Tequatrovirus T4 |
2.4.1.26 | partial, E. coli infected with | Escherichia phage T2 |
2.4.1.26 | partial, E. coli infected with | Enterobacteria phage T6 |
2.4.1.28 | partial, using streptomycin sulfate treatment and fractionation on DEAE-cellulose | Enterobacteria phage T6 |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.26 | additional information | - |
- |
Tequatrovirus T4 |
2.4.1.26 | additional information | - |
- |
Escherichia phage T2 |
2.4.1.26 | additional information | - |
- |
Enterobacteria phage T6 |
2.4.1.28 | 0.0017 | - |
crude extract | Enterobacteria phage T6 |
2.4.1.28 | 0.0067 | - |
purified enzyme | Enterobacteria phage T6 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.26 | UDPglucose + 5-hydroxymethylcytosine containing DNA | - |
Escherichia phage T2 | UDP + alpha-glucosyl-5-hydroxymethylcytosine containing DNA | - |
? | |
2.4.1.26 | UDPglucose + 5-hydroxymethylcytosine containing DNA | extent of glucosylation varies between 50% and 80% of the amount of hydroxymethylcytosine residues calculated to be present, T4 DNA with no unsubstituted hydroxymethylcytosine groups does not serve as acceptor, T2 DNA: no activity with enzyme from T2-infected cells, small activity with enzyme from T4- and T6-infected cells | Tequatrovirus T4 | UDP + alpha-glucosyl-5-hydroxymethylcytosine containing DNA | - |
? | |
2.4.1.26 | UDPglucose + 5-hydroxymethylcytosine containing DNA | extent of glucosylation varies between 50% and 80% of the amount of hydroxymethylcytosine residues calculated to be present, T4 DNA with no unsubstituted hydroxymethylcytosine groups does not serve as acceptor, T2 DNA: no activity with enzyme from T2-infected cells, small activity with enzyme from T4- and T6-infected cells | Enterobacteria phage T6 | UDP + alpha-glucosyl-5-hydroxymethylcytosine containing DNA | - |
? | |
2.4.1.28 | UDP-glucose + glucosyl-5-hydroxymethylcytosine residue in DNA | no transfer of glucose to synthetic 5-hydroxymethylcytosine-DNA or to T6 DNA, enzyme glucosylates glucosylhydroxymethylcytosine residues of either alpha or beta configuration | Enterobacteria phage T6 | UDP + beta-D-glucosyl-beta-D-glucosyl-5-hydroxymethylcytosine containing DNA | - |
? | |
2.4.1.28 | UDP-glucose + glucosylhydroxymethylcytosine residue in DNA | - |
Enterobacteria phage T6 | UDP + beta-D-glucosyl-beta-D-glucosyl-hydroxymethylcytosine containing DNA | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.28 | 30 | - |
assay at | Enterobacteria phage T6 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.28 | 7.5 | - |
assay at | Enterobacteria phage T6 |