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Literature summary extracted from

  • Kornberg, S.R.; Zimmerman, S.B.; Kornberg, A.
    Glucosylation of deoxyribonucleic acid by enzymes from bacteriophage-infected Escherichia coli (1961), J. Biol. Chem., 236, 1487-1493.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.26 phosphate buffer stimulates Tequatrovirus T4
2.4.1.26 sulfhydryl reagents required Enterobacteria phage T6
2.4.1.28 sulfhydryl reagents required Enterobacteria phage T6

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.26 MgCl2
-
Enterobacteria phage T6
2.4.1.26 MgCl2 stimulates Tequatrovirus T4
2.4.1.28 Mg2+ required for activity Enterobacteria phage T6

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.28 UDP-glucose + glucosylhydroxymethylcytosine residue in DNA Enterobacteria phage T6
-
UDP + beta-D-glucosyl-beta-D-glucosyl-hydroxymethylcytosine containing DNA
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.26 Enterobacteria phage T6
-
Escherichia coli infected with
-
2.4.1.26 Escherichia phage T2
-
Escherichia coli infected with
-
2.4.1.26 Tequatrovirus T4
-
Escherichia coli infected with
-
2.4.1.28 Enterobacteria phage T6
-
Escherichia coli infected with
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.26 partial, E. coli infected with Tequatrovirus T4
2.4.1.26 partial, E. coli infected with Escherichia phage T2
2.4.1.26 partial, E. coli infected with Enterobacteria phage T6
2.4.1.28 partial, using streptomycin sulfate treatment and fractionation on DEAE-cellulose Enterobacteria phage T6

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.26 additional information
-
-
Tequatrovirus T4
2.4.1.26 additional information
-
-
Escherichia phage T2
2.4.1.26 additional information
-
-
Enterobacteria phage T6
2.4.1.28 0.0017
-
crude extract Enterobacteria phage T6
2.4.1.28 0.0067
-
purified enzyme Enterobacteria phage T6

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.26 UDPglucose + 5-hydroxymethylcytosine containing DNA
-
Escherichia phage T2 UDP + alpha-glucosyl-5-hydroxymethylcytosine containing DNA
-
?
2.4.1.26 UDPglucose + 5-hydroxymethylcytosine containing DNA extent of glucosylation varies between 50% and 80% of the amount of hydroxymethylcytosine residues calculated to be present, T4 DNA with no unsubstituted hydroxymethylcytosine groups does not serve as acceptor, T2 DNA: no activity with enzyme from T2-infected cells, small activity with enzyme from T4- and T6-infected cells Tequatrovirus T4 UDP + alpha-glucosyl-5-hydroxymethylcytosine containing DNA
-
?
2.4.1.26 UDPglucose + 5-hydroxymethylcytosine containing DNA extent of glucosylation varies between 50% and 80% of the amount of hydroxymethylcytosine residues calculated to be present, T4 DNA with no unsubstituted hydroxymethylcytosine groups does not serve as acceptor, T2 DNA: no activity with enzyme from T2-infected cells, small activity with enzyme from T4- and T6-infected cells Enterobacteria phage T6 UDP + alpha-glucosyl-5-hydroxymethylcytosine containing DNA
-
?
2.4.1.28 UDP-glucose + glucosyl-5-hydroxymethylcytosine residue in DNA no transfer of glucose to synthetic 5-hydroxymethylcytosine-DNA or to T6 DNA, enzyme glucosylates glucosylhydroxymethylcytosine residues of either alpha or beta configuration Enterobacteria phage T6 UDP + beta-D-glucosyl-beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
-
?
2.4.1.28 UDP-glucose + glucosylhydroxymethylcytosine residue in DNA
-
Enterobacteria phage T6 UDP + beta-D-glucosyl-beta-D-glucosyl-hydroxymethylcytosine containing DNA
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.28 30
-
assay at Enterobacteria phage T6

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.28 7.5
-
assay at Enterobacteria phage T6