Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Imamura, H.; Fushinobu, S.; Yamamoto, M.; Kumasaka, T.; Jeon, B.S.; Wakagi, T.; Matsuzawa, H.
    Crystal structures of 4-alpha-glucanotransferase from Thermococcus litoralis and its complex with an inhibitor (2003), J. Biol. Chem., 278, 19378-19386.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.25 crystallized in 2 forms, I and II, form I crystals belongs to hexagonal space group P6(4)22, form II crystals to orthorhombic space group P2(1)2(1)2 Thermococcus litoralis
2.4.1.25 hanging drop vapor diffusion method at 25°C, crystal structure of the enzyme with and without an inhibitor, acarbose. The acarbose-bound structure clarifies that Glu123 and Asp214 are the catalytic nucleophile and acid/base catalyst, respectively Thermococcus litoralis

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.25 D214N the specific activity of the D214N mutant is decreased about 10000fold as compared with that of the wild-type enzyme Thermococcus litoralis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.25 acarbose
-
 Thermococcus litoralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.25 78000
-
 2 * 78000, calculated from sequence Thermococcus litoralis
2.4.1.25 168000
-
 gel filtration Thermococcus litoralis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Dictyoglomus thermophilum
-
-
-
2.4.1.25 Pyrococcus furiosus
-
-
-
2.4.1.25 Solanum tuberosum
-
-
-
2.4.1.25 Thermococcus kodakarensis
-
-
-
2.4.1.25 Thermococcus litoralis
-
-
-
2.4.1.25 Thermococcus litoralis O32462
-
-
2.4.1.25 Thermococcus litoralis DSM 5473 O32462
-
-
2.4.1.25 Thermus aquaticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25
-
 Thermococcus litoralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 amylose + maltopentaose
-
 Thermococcus kodakarensis cyclic alpha-1,4-glucan
-
 ?
2.4.1.25 amylose + maltopentaose
-
 Thermus aquaticus cyclic alpha-1,4-glucan cycloamylose ?
2.4.1.25 amylose + maltopentaose
-
 Solanum tuberosum cyclic alpha-1,4-glucan cycloamylose ?
2.4.1.25 amylose + maltopentaose
-
 Thermococcus litoralis cyclic alpha-1,4-glucan cycloamylose ?
2.4.1.25 maltose + maltose key role in maltose metabolism Thermococcus litoralis maltotriose + glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.25 homodimer 2 * 78000, calculated from sequence Thermococcus litoralis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.25 TLGT
-
 Thermococcus litoralis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.25 80
-
 assay at Thermococcus litoralis