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Literature summary extracted from

  • Kitahata, S.; Murakami, H.; Okada, S.
    Purification and some properties of amylomaltase from Escherichia coli IFO 3806 (1989), Agric. Biol. Chem., 53, 2653-2659.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.25 38000
-
 1 * 38000 + 1 * 47000, amylomaltase Pseudomonas stutzeri
2.4.1.25 47000
-
 1 * 38000 + 1 * 47000, amylomaltase Pseudomonas stutzeri
2.4.1.25 71000
-
 strain ML 308 Escherichia coli
2.4.1.25 74000
-
-
 Pseudomonas stutzeri
2.4.1.25 93000
-
 SDS-PAGE Escherichia coli
2.4.1.25 124000
-
 strain Ml 30 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.25 maltopentaose + maltopentaose Escherichia coli metabolism of starch in the bacterium maltooligosaccharides
-
 ?
2.4.1.25 maltopentaose + maltopentaose Pseudomonas stutzeri metabolism of starch in the bacterium maltooligosaccharides
-
 ?
2.4.1.25 maltopentaose + maltopentaose Pseudomonas stutzeri NRRL B3389 metabolism of starch in the bacterium maltooligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Escherichia coli
-
-
-
2.4.1.25 Escherichia coli
-
 Ml 308
-
2.4.1.25 Escherichia coli
-
 IFO 3806
-
2.4.1.25 Escherichia coli ML 30
-
-
-
2.4.1.25 Escherichia coli ML 308
-
 Ml 308
-
2.4.1.25 Pseudomonas stutzeri
-
 NRRL B3389
-
2.4.1.25 Pseudomonas stutzeri NRRL B3389
-
 NRRL B3389
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 maltodextrin + maltodextrin
-
 Escherichia coli maltooligosaccharides
-
 ?
2.4.1.25 maltodextrin + maltodextrin
-
 Escherichia coli ML 308 maltooligosaccharides
-
 ?
2.4.1.25 maltodextrin + maltodextrin
-
 Escherichia coli ML 30 maltooligosaccharides
-
 ?
2.4.1.25 maltopentaitol
-
 Escherichia coli maltohexaitol + ?
-
 ?
2.4.1.25 maltopentaitol
-
 Escherichia coli ML 308 maltohexaitol + ?
-
 ?
2.4.1.25 maltopentaitol
-
 Escherichia coli ML 30 maltohexaitol + ?
-
 ?
2.4.1.25 maltopentaose + maltopentaose metabolism of starch in the bacterium Escherichia coli maltooligosaccharides
-
 ?
2.4.1.25 maltopentaose + maltopentaose metabolism of starch in the bacterium Pseudomonas stutzeri maltooligosaccharides
-
 ?
2.4.1.25 maltopentaose + maltopentaose metabolism of starch in the bacterium Pseudomonas stutzeri NRRL B3389 maltooligosaccharides
-
 ?
2.4.1.25 maltosylsucrose
-
 Escherichia coli ?
-
 ?
2.4.1.25 maltosylsucrose
-
 Escherichia coli ML 308 ?
-
 ?
2.4.1.25 maltosylsucrose
-
 Escherichia coli ML 30 ?
-
 ?
2.4.1.25 maltotetraitol
-
 Escherichia coli maltopentaitol + ?
-
 ?
2.4.1.25 maltotetraitol
-
 Escherichia coli ML 308 maltopentaitol + ?
-
 ?
2.4.1.25 maltotetraitol
-
 Escherichia coli ML 30 maltopentaitol + ?
-
 ?
2.4.1.25 additional information does not act on maltitol, maltotriitol, glucosylsucrose, isomaltose, panose, isopanose or isomaltosylmaltose, enzyme does not catalyze hydrolytic action on maltotetraitol, maltopentaitol or maltosylsucrose, sorbitol and maltitol are not produced Escherichia coli ?
-
 ?
2.4.1.25 additional information does not act on maltitol, maltotriitol, glucosylsucrose, isomaltose, panose, isopanose or isomaltosylmaltose, enzyme does not catalyze hydrolytic action on maltotetraitol, maltopentaitol or maltosylsucrose, sorbitol and maltitol are not produced Escherichia coli ML 308 ?
-
 ?
2.4.1.25 additional information does not act on maltitol, maltotriitol, glucosylsucrose, isomaltose, panose, isopanose or isomaltosylmaltose, enzyme does not catalyze hydrolytic action on maltotetraitol, maltopentaitol or maltosylsucrose, sorbitol and maltitol are not produced Escherichia coli ML 30 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.25 dimer 1 * 38000 + 1 * 47000, amylomaltase Pseudomonas stutzeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.25 35
-
-
 Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.25 30 55
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.25 45
-
 stable up to Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.25 6.5
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.25 4 9
-
 Escherichia coli