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Literature summary extracted from

  • Kakefuda, G.; Duke, S.H.
    Characterization of pea chloroplast D-enzyme (4-alpha-D-glucanotransferase) (1989), Plant Physiol., 91, 136-143.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.4.1.25 unstable to freezing, glycerol reduces activity and cannot be used to stabilize the enzyme at freezing temperatures Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.25 3.3
-
 maltotriose
-
 Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.25 chloroplast
-
 Spinacia oleracea 9507
-
2.4.1.25 chloroplast
-
 Pisum sativum 9507
-
2.4.1.25 chloroplast
-
 Arabidopsis thaliana 9507
-
2.4.1.25 cytosol
-
 Pisum sativum 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.25 50000
-
 2 * 50000, SDS-PAGE Pisum sativum
2.4.1.25 98000
-
 gel filtration Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.25 maltopentaose + maltopentaose Escherichia coli metabolism of starch in the bacterium maltooligosaccharides
-
 ?
2.4.1.25 maltopentaose + maltopentaose Paenibacillus macerans metabolism of starch in the bacterium maltooligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Arabidopsis thaliana
-
-
-
2.4.1.25 Bacillus subtilis
-
-
-
2.4.1.25 Bos taurus
-
 bovine
-
2.4.1.25 Daucus carota
-
 carrot
-
2.4.1.25 Escherichia coli
-
-
-
2.4.1.25 Paenibacillus macerans
-
-
-
2.4.1.25 Pisum sativum
-
 pea
-
2.4.1.25 Pisum sativum
-
 L. cv. Laxton's progress No. 9
-
2.4.1.25 Solanum lycopersicum
-
 tomato
-
2.4.1.25 Solanum tuberosum
-
 potato
-
2.4.1.25 Spinacia oleracea
-
 spinach
-
2.4.1.25 Triticum aestivum
-
 wheat
-
2.4.1.25 Vigna radiata var. radiata
-
-
-
2.4.1.25 Zea mays
-
 sweet corn
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25
-
 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.25 blood
-
 Bos taurus
-
2.4.1.25 fruit
-
 Solanum lycopersicum
-
2.4.1.25 germ
-
 Triticum aestivum
-
2.4.1.25 kernel
-
 Zea mays
-
2.4.1.25 kernel
-
 Arabidopsis thaliana
-
2.4.1.25 leaf
-
 Spinacia oleracea
-
2.4.1.25 leaf
-
 Solanum tuberosum
-
2.4.1.25 leaf
-
 Arabidopsis thaliana
-
2.4.1.25 root
-
 Solanum tuberosum
-
2.4.1.25 root
-
 Daucus carota
-
2.4.1.25 stipule
-
 Pisum sativum
-
2.4.1.25 tuber
-
 Solanum tuberosum
-
2.4.1.25 tuber
-
 Arabidopsis thaliana
-
2.4.1.25 tuber
-
 Solanum lycopersicum
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.25 0°C, unstable to freezing Pisum sativum
2.4.1.25 4°C, stable for several months Pisum sativum
2.4.1.25 4°C, very stable over long periods Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 maltohexaose + maltotriose
-
 Pisum sativum maltodextrins
-
 ?
2.4.1.25 maltopentaose + maltopentaose metabolism of starch in the bacterium Escherichia coli maltooligosaccharides
-
 ?
2.4.1.25 maltopentaose + maltopentaose metabolism of starch in the bacterium Paenibacillus macerans maltooligosaccharides
-
 ?
2.4.1.25 maltopentaose + maltotriose
-
 Pisum sativum maltodextrins
-
 ?
2.4.1.25 maltotetraose + maltotriose
-
 Pisum sativum maltodextrins
-
 ?
2.4.1.25 maltotetraose + maltotriose
-
 Solanum tuberosum maltodextrins
-
 ?
2.4.1.25 maltotheptaose + maltotriose
-
 Pisum sativum maltodextrins
-
 ?
2.4.1.25 maltotriose + maltotriose
-
 Pisum sativum maltopentaose + D-glucose
-
 ?
2.4.1.25 maltotriose + maltotriose
-
 Arabidopsis thaliana maltopentaose + D-glucose
-
 ?
2.4.1.25 maltotriose + maltotriose
-
 Pisum sativum maltodextrins
-
 ?
2.4.1.25 additional information
-
 Escherichia coli ?
-
 ?
2.4.1.25 additional information
-
 Arabidopsis thaliana ?
-
 ?
2.4.1.25 additional information major pathway for starch degradation in chloroplasts Pisum sativum ?
-
 ?
2.4.1.25 additional information cannot use maltose or cyclohexaamylose as a substrate Vigna radiata var. radiata ?
-
 ?
2.4.1.25 additional information cannot use maltose or cyclohexaamylose as a substrate Pisum sativum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.25 dimer 2 * 50000, SDS-PAGE Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.25 5.2
-
-
 Pisum sativum
2.4.1.25 5.5
-
-
 Daucus carota
2.4.1.25 5.5
-
-
 Solanum lycopersicum
2.4.1.25 6.5
-
-
 Escherichia coli
2.4.1.25 6.5
-
-
 Arabidopsis thaliana
2.4.1.25 6.7
-
-
 Solanum tuberosum
2.4.1.25 7.5 8
-
 Pisum sativum