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Literature summary extracted from

  • Taniguchi, N.; Yanagisawa, K.; Makita, A.; Naiki, M.
    Purification and properties of rat liver globotriaosylceramide synthase, UDP-galactose:lactosylceramide alpha1-4-galactosyltransferase (1985), J. Biol. Chem., 260, 4908-4913.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.228 0.006
-
 lactosylceramide
-
 Rattus norvegicus
2.4.1.228 0.03
-
 UDP-galactose
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.228 Cu2+ some activity Rattus norvegicus
2.4.1.228 Mn2+ a second divalent cation in the enzyme assay can inhibit the enzyme activity Rattus norvegicus
2.4.1.228 Ni2+ some activity Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.228 22000
-
 alpha 1 * 65000 + beta 1 * 22000, linked through a disulfide bond, SDS-PAGE Rattus norvegicus
2.4.1.228 65000
-
 alpha 1 * 65000 + beta 1 * 22000, linked through a disulfide bond, SDS-PAGE Rattus norvegicus
2.4.1.228 90000
-
 corresponding to the Stokes radius of 40 A, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.228 UDP-galactose + beta-D-galactosyl-(1-4)-D-glucosylceramide Rattus norvegicus
-
 UDP + alpha-D-galactosyl-(1-4)-beta-D-galactosyl-(1-4)-D-glucosylceramide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.228 Rattus norvegicus
-
 male Wistar rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.228
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.228 liver stored at -80°C until use Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.228 0.00458
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.4.1.228 -20°C, in 20% glycerol, stored at a concentration greater than 0.1 mg/ml without detectable loss of activity upon repeated freezing and thawing for 3 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.228 additional information no substrates: galactosylceramide, glucosylceramide, neolactotetraose, neolactotetraosylceramide Rattus norvegicus ?
-
 ?
2.4.1.228 UDP-galactose + beta-D-galactosyl-(1-4)-D-glucosylceramide
-
 Rattus norvegicus UDP + alpha-D-galactosyl-(1-4)-beta-D-galactosyl-(1-4)-D-glucosylceramide
-
 ?
2.4.1.228 UDP-galactose + lactose
-
 Rattus norvegicus ?
-
 ?
2.4.1.228 UDP-galactose + lactosylceramide UDP-galactose is the sole sugar donor Rattus norvegicus UDP + globotriaosylceramide
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.228 dimer alpha 1 * 65000 + beta 1 * 22000, linked through a disulfide bond, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.228 37
-
 enzyme assay Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.228 7.2
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.228 6.7 7.4
-
 Rattus norvegicus