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Literature summary extracted from

  • Lidholt, K.; Lindahl, U.
    Biosynthesis of heparin. The D-glucuronosyl- and N-acetyl-D-glucosaminyltransferase reactions and their relation to polymer modification (1992), Biochem. J., 287, 21-29.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.224 0.006
-
 [D-glucuronic acid-N-acetyl-glucosamine]14 -D-glucuronic acid-2,5-anhydro-D-mannose
-
 Mus musculus
2.4.1.224 0.06
-
 [D-glucuronic acid-N-acetyl-glucosamine]4 -D-glucuronic acid-2,5-anhydro-D-mannose
-
 Mus musculus
2.4.1.224 0.4
-
 UDP-N-acetyl-D-glucosamine
-
 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.224 microsome
-
 Mus musculus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.224 Mn2+ required, can not be substituted by other divalent cations Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.224 UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan Mus musculus elongation of growing chains of heparin and heparan sulfate, tumor suppressor UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan Bos taurus elongation of growing chains of heparin and heparan sulfate, tumor suppressor UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.224 Bos taurus
-
-
-
2.4.1.224 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.224
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.224 fibroblast
-
 Mus musculus
-
2.4.1.224 mastocytoma cell
-
 Mus musculus
-
2.4.1.224 serum
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.224 additional information EXT1 and EXT2 proteins also acts as beta(1,4)-glucuronyltransferase (EC 2.4.1.225) Mus musculus ?
-
 ?
2.4.1.224 additional information EXT1 and EXT2 proteins also acts as beta(1,4)-glucuronyltransferase (EC 2.4.1.225) Bos taurus ?
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 Mus musculus UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 Bos taurus UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan elongation of growing chains of heparin and heparan sulfate, tumor suppressor Mus musculus UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan elongation of growing chains of heparin and heparan sulfate, tumor suppressor Bos taurus UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + [D-glucuronic acid-N-acetyl-glucosamine]14 -D-glucuronic acid-2,5-anhydro-D-mannose
-
 Mus musculus ?
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + [D-glucuronic acid-N-acetyl-glucosamine]4 -D-glucuronic acid-2,5-anhydro-D-mannose
-
 Mus musculus ?
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + [D-glucuronosyl-beta-(1->4)-N-acetyl-D-glucoseamin-alpha-(1->4)]n-D-glucuronosyl-2,5-anhydro-D-mannose substrate is produced by E. coli K5, acceptor ability increases with increasing chain length Mus musculus UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-[D-glucuronosyl-beta-(1->4)-N-acetyl-D-glucoseaminyl-alpha-(1->4)]n-D-glucuronosyl-2,5-anhydro-D-mannose
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + [D-glucuronosyl-beta-(1->4)-N-acetyl-D-glucoseamin-alpha-(1->4)]n-D-glucuronosyl-2,5-anhydro-D-mannose substrate is produced by E. coli K5, acceptor ability increases with increasing chain length Bos taurus UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-[D-glucuronosyl-beta-(1->4)-N-acetyl-D-glucoseaminyl-alpha-(1->4)]n-D-glucuronosyl-2,5-anhydro-D-mannose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.224 6 7.2 broad optimum Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.224 5.2 8.4
-
 Mus musculus