Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Marcinek, H.; Weyler, W.; Deus-Neumann, B.; Zenk, M.H.
    Indoxyl-UDPG-glucosyltransferase from Baphicacanthus cusia (2000), Phytochemistry, 53, 201-207.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.220 0.5
-
 5-hydroxyindole
-
 Strobilanthes cusia
2.4.1.220 1.2
-
 indoxyl
-
 Strobilanthes cusia
2.4.1.220 1.4
-
 7-hydroxyindole
-
 Strobilanthes cusia
2.4.1.220 1.7
-
 UDP-glucose
-
 Strobilanthes cusia
2.4.1.220 1.9
-
 4-hydroxyindole
-
 Strobilanthes cusia
2.4.1.220 3.3
-
 6-hydroxyindole
-
 Strobilanthes cusia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.220 60000
-
 SDS-PAGE Strobilanthes cusia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.220 UDP-glucose + indoxyl Strobilanthes cusia indole is the biosynthetic precursor to the indoxyl derivates indican isatan B UDP + 1H-indole-3-yl-beta-D-glucopyranoside i.e. indican ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.220 Strobilanthes cusia
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.4.1.220 anaerobic conditions, since under aerobic conditions indoxyl dimerizes spontaneously to indigo Strobilanthes cusia

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.220
-
 Strobilanthes cusia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.220 leaf
-
 Strobilanthes cusia
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.220 additional information
-
-
 Strobilanthes cusia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.220 additional information not: 2-OH-indole Strobilanthes cusia ?
-
 ?
2.4.1.220 UDP-glucose + 4-hydroxyindole minimal activity Strobilanthes cusia UDP + 1H-indol-4-yl-beta-D-glucopyranoside
-
 ?
2.4.1.220 UDP-glucose + 5-hydroxyindole activity as 3-OH-indole Strobilanthes cusia UDP + 1H-indol-5-yl-beta-D-glucopyranoside
-
 ?
2.4.1.220 UDP-glucose + 6-hydroxyindole minimal activity Strobilanthes cusia UDP + 1H-indol-6-yl-beta-D-glucopyranoside
-
 ?
2.4.1.220 UDP-glucose + 7-hydroxyindole
-
 Strobilanthes cusia UDP + 1H-indol-7-yl-beta-D-glucopyranoside
-
 ?
2.4.1.220 UDP-glucose + indoxyl best substrate Strobilanthes cusia UDP + 1H-indole-3-yl-beta-D-glucopyranoside i.e. indican ?
2.4.1.220 UDP-glucose + indoxyl i.e. indole-3-ol or 3-OH-indole Strobilanthes cusia UDP + 1H-indole-3-yl-beta-D-glucopyranoside i.e. indican ?
2.4.1.220 UDP-glucose + indoxyl indole is the biosynthetic precursor to the indoxyl derivates indican isatan B Strobilanthes cusia UDP + 1H-indole-3-yl-beta-D-glucopyranoside i.e. indican ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.220 30
-
-
 Strobilanthes cusia
2.4.1.220 45 48
-
 Strobilanthes cusia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.220 additional information
-
 additional information
-
 Strobilanthes cusia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.220 8.5
-
 pI: 6.5 Strobilanthes cusia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.220 6.8 10.2 6.8 and 10.2 half-maximal-activity Strobilanthes cusia