EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.125 | pharmacology | the RG product vomilenine is a direct intermediate in ajmaline biosynthesis and utilization of raucaffricine for the ajmaline biosynthestic pathway could be a crucial and rate-limiting step in the formation of the antiarrythmic drug ajmaline | Rauvolfia serpentina |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.219 | expression in Escherichia coli | Rauvolfia serpentina |
3.2.1.125 | expression in Escherichia coli TOP 10, sequencing | Rauvolfia serpentina |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.219 | 1.3 | - |
Raucaffricine | - |
Rauvolfia serpentina | |
2.4.1.219 | 1.8 | - |
strictosidine | - |
Rauvolfia serpentina | |
3.2.1.125 | 1.3 | - |
Raucaffricine | pH 5, 28°C, recombinant RG | Rauvolfia serpentina | |
3.2.1.125 | 1.8 | - |
strictosidine | pH 5, 28°C, recombinant RG | Rauvolfia serpentina |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.125 | 60931 | - |
x * 60931, sequence calculation, x * 61000, SDS-PAGE | Rauvolfia serpentina |
3.2.1.125 | 61000 | - |
x * 60931, sequence calculation, x * 61000, SDS-PAGE | Rauvolfia serpentina |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.219 | UDP-glucose + vomilenine | Rauvolfia serpentina | - |
UDP + raucaffricine | - |
r | |
3.2.1.125 | raucaffricine + H2O | Rauvolfia serpentina | specifically involved in alkaloid biosynthesis, e.g. ajmaline | D-glucose + vomilenine | vomilene is a direct intermediate in ajmaline biosynthesis | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.219 | Rauvolfia serpentina | - |
- |
- |
3.2.1.125 | Rauvolfia serpentina | Q9SPP9 | BENTH. ex KURZ | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.125 | native and recombinant RG | Rauvolfia serpentina |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.125 | cell suspension culture | - |
Rauvolfia serpentina | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.219 | additional information | - |
0.5 µkat/mg | Rauvolfia serpentina |
2.4.1.219 | additional information | - |
2.6 nkat/mg | Rauvolfia serpentina |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.219 | strictosidine + UDP | recombinant enzyme | Rauvolfia serpentina | ? | - |
? | |
2.4.1.219 | UDP-glucose + vomilenine | - |
Rauvolfia serpentina | UDP + raucaffricine | - |
r | |
3.2.1.125 | 1,2-dihydroraucaffricine + H2O | recombinant RG | Rauvolfia serpentina | ? | - |
? | |
3.2.1.125 | 1-methyl-1,2-dihydroraucaffricine + H2O | recombinant RG | Rauvolfia serpentina | ? | - |
? | |
3.2.1.125 | 21-glucopyranosyl-hydroxysarpagan-17-al + H2O | recombinant RG | Rauvolfia serpentina | ? | - |
? | |
3.2.1.125 | 21-glucopyranosyl-hydroxysarpagan-17-ol + H2O | recombinant RG | Rauvolfia serpentina | ? | - |
? | |
3.2.1.125 | 5alpha-carboxystrictosidine + H2O | recombinant RG | Rauvolfia serpentina | ? | - |
? | |
3.2.1.125 | raucaffricine + H2O | best substrate, highly specific enzyme | Rauvolfia serpentina | D-glucose + vomilenine | - |
? | |
3.2.1.125 | raucaffricine + H2O | specifically involved in alkaloid biosynthesis, e.g. ajmaline | Rauvolfia serpentina | D-glucose + vomilenine | vomilene is a direct intermediate in ajmaline biosynthesis | ? | |
3.2.1.125 | strictosidine + H2O | only recombinant RG, no substrate of native RG | Rauvolfia serpentina | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.125 | ? | x * 60931, sequence calculation, x * 61000, SDS-PAGE | Rauvolfia serpentina |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.125 | More | belongs to family1 of glycosyl hydrolases | Rauvolfia serpentina |
3.2.1.125 | RG | - |
Rauvolfia serpentina |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.125 | 28 | - |
assay at | Rauvolfia serpentina |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.125 | 5 | - |
assay at | Rauvolfia serpentina |