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Literature summary extracted from

  • Warzecha, H.; Gerasimenko, I.; Kutchan, T.M.; Stockigt, J.
    Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis (2000), Phytochemistry, 54, 657-666.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.125 pharmacology the RG product vomilenine is a direct intermediate in ajmaline biosynthesis and utilization of raucaffricine for the ajmaline biosynthestic pathway could be a crucial and rate-limiting step in the formation of the antiarrythmic drug ajmaline Rauvolfia serpentina

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.219 expression in Escherichia coli Rauvolfia serpentina
3.2.1.125 expression in Escherichia coli TOP 10, sequencing Rauvolfia serpentina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.219 1.3
-
Raucaffricine
-
Rauvolfia serpentina
2.4.1.219 1.8
-
strictosidine
-
Rauvolfia serpentina
3.2.1.125 1.3
-
Raucaffricine pH 5, 28°C, recombinant RG Rauvolfia serpentina
3.2.1.125 1.8
-
strictosidine pH 5, 28°C, recombinant RG Rauvolfia serpentina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.125 60931
-
x * 60931, sequence calculation, x * 61000, SDS-PAGE Rauvolfia serpentina
3.2.1.125 61000
-
x * 60931, sequence calculation, x * 61000, SDS-PAGE Rauvolfia serpentina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.219 UDP-glucose + vomilenine Rauvolfia serpentina
-
UDP + raucaffricine
-
r
3.2.1.125 raucaffricine + H2O Rauvolfia serpentina specifically involved in alkaloid biosynthesis, e.g. ajmaline D-glucose + vomilenine vomilene is a direct intermediate in ajmaline biosynthesis ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.219 Rauvolfia serpentina
-
-
-
3.2.1.125 Rauvolfia serpentina Q9SPP9 BENTH. ex KURZ
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.125 native and recombinant RG Rauvolfia serpentina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.125 cell suspension culture
-
Rauvolfia serpentina
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.219 additional information
-
0.5 µkat/mg Rauvolfia serpentina
2.4.1.219 additional information
-
2.6 nkat/mg Rauvolfia serpentina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.219 strictosidine + UDP recombinant enzyme Rauvolfia serpentina ?
-
?
2.4.1.219 UDP-glucose + vomilenine
-
Rauvolfia serpentina UDP + raucaffricine
-
r
3.2.1.125 1,2-dihydroraucaffricine + H2O recombinant RG Rauvolfia serpentina ?
-
?
3.2.1.125 1-methyl-1,2-dihydroraucaffricine + H2O recombinant RG Rauvolfia serpentina ?
-
?
3.2.1.125 21-glucopyranosyl-hydroxysarpagan-17-al + H2O recombinant RG Rauvolfia serpentina ?
-
?
3.2.1.125 21-glucopyranosyl-hydroxysarpagan-17-ol + H2O recombinant RG Rauvolfia serpentina ?
-
?
3.2.1.125 5alpha-carboxystrictosidine + H2O recombinant RG Rauvolfia serpentina ?
-
?
3.2.1.125 raucaffricine + H2O best substrate, highly specific enzyme Rauvolfia serpentina D-glucose + vomilenine
-
?
3.2.1.125 raucaffricine + H2O specifically involved in alkaloid biosynthesis, e.g. ajmaline Rauvolfia serpentina D-glucose + vomilenine vomilene is a direct intermediate in ajmaline biosynthesis ?
3.2.1.125 strictosidine + H2O only recombinant RG, no substrate of native RG Rauvolfia serpentina ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.125 ? x * 60931, sequence calculation, x * 61000, SDS-PAGE Rauvolfia serpentina

Synonyms

EC Number Synonyms Comment Organism
3.2.1.125 More belongs to family1 of glycosyl hydrolases Rauvolfia serpentina
3.2.1.125 RG
-
Rauvolfia serpentina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.125 28
-
assay at Rauvolfia serpentina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.125 5
-
assay at Rauvolfia serpentina