EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.21 | - |
Escherichia coli |
2.4.1.21 | expression of full-length and truncated enzyme in Escherichia coli | Zea mays |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.21 | additional information | - |
additional information | - |
Escherichia coli | |
2.4.1.21 | additional information | - |
additional information | recombinant starch synthase I, amylopectin: 0.24 mg/ml, glycogen: 0.041 mg/ml | Zea mays | |
2.4.1.21 | 0.18 | - |
ADPglucose | starch synthase I, presence of 500 mM citrate | Zea mays | |
2.4.1.21 | 0.24 | - |
ADPglucose | starch synthase I, absence of citrate | Zea mays |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.21 | starch grain | - |
Zea mays | 43036 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.21 | Escherichia coli | - |
- |
- |
2.4.1.21 | Zea mays | - |
starch synthase I | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.21 | recombinant starch synthase I, ammonium sulfate, amylose column, Mono Q | Zea mays |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.21 | 0.06 | - |
unprimed reaction in the presence of 500 mM citrate, recombinant starch synthase I | Zea mays |
2.4.1.21 | 12.2 | - |
primed reaction, recombinant starch synthase I | Zea mays |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.21 | 40 | 50 | recombinant starch synthase I in the presence of citrate | Zea mays |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.21 | 8 | 10.3 | starch synthase II, sharp decline in activity below pH 7.0 | Zea mays |