Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ozbun, J.L.; Hawker, J.S.; Preiss, J.
    Soluble adenosine diphosphate glucose alpha-1,4-glucan alpha-4-glucosyltransferases from spinach leaves (1972), Biochem. J., 126, 953-963.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.21 additional information
-
 additional information amylose: 0.02-0.06 mg/ml, isoenzymes I, II and IV, amylopectin: 0.03-0.05 mg/ml, isoenzymes I, II and IV, rabbit liver glycogen: 0.8-1.02 mg/ml, isoenzymes I, II and IV, oyster glycogen: 1.18-6.66 mg/ml, isoenzymes I-IV, amylopectin beta-limit dextrin: 0.02-0.22 mg/ml, isoenzymes I-IV, glycogen E. coli beta-limit dextrin: 1.0-2.0 mg/ml, isoenzymes I-IV Spinacia oleracea
2.4.1.21 0.15
-
 ADPglucose isoenzyme III Spinacia oleracea
2.4.1.21 0.2
-
 ADPglucose isoenzyme I Spinacia oleracea
2.4.1.21 0.25
-
 ADPglucose isoenzyme IV Spinacia oleracea
2.4.1.21 0.29
-
 ADPglucose isoenzyme II Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.21 soluble
-
 Spinacia oleracea
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.21 Spinacia oleracea
-
 isoenzymes I-IV
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.21 4 isoenzymes, ammonium sulfate, DEAE-cellulose Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.21 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.21 0.032
-
 primed reaction, isoenzyme I Spinacia oleracea
2.4.1.21 0.106
-
 primed reaction, isoenzyme IV Spinacia oleracea
2.4.1.21 0.118
-
 primed reaction, isoenzyme III Spinacia oleracea
2.4.1.21 0.28
-
 unprimed reaction, isoenzyme III Spinacia oleracea
2.4.1.21 0.338
-
 primed reaction, isoenzyme II Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan citrate independent enzyme requires a glucan primer e.g. rabbit liver glycogen Spinacia oleracea ADP + alpha-1,4-polyglucan
-
 ?
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan glucosyl acceptor: oyster glycogen Spinacia oleracea ADP + alpha-1,4-polyglucan
-
 ?
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan glucosyl acceptors: amylopectin beta-limit dextrin, glycogen E. coli beta-limit dextrin, maltose and maltotriose Spinacia oleracea ADP + alpha-1,4-polyglucan
-
 ?
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan 4 isoenzymes, isoenzyme III shows unprimed activity, no activity with UDPglucose and glucose 1-phosphate Spinacia oleracea ADP + alpha-1,4-polyglucan
-
 ?
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan glucosyl acceptor: amylose Spinacia oleracea ADP + alpha-1,4-polyglucan
-
 ?
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan glucosyl acceptor: amylopectin Spinacia oleracea ADP + alpha-1,4-polyglucan
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.21 30
-
 4 isozymes, primed reaction Spinacia oleracea
2.4.1.21 37
-
 unprimed synthase activity of isoenzyme III Spinacia oleracea

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.21 22 44
-
 Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.21 7.5 8.5 isoenzymes I-IV Spinacia oleracea