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Literature summary extracted from

  • Pollock, C.; Preiss, J.
    The citrate-stimulated starch synthase of starchy maize kernels: purification and properties (1980), Arch. Biochem. Biophys., 204, 578-588.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.21 ammonium sulfate 500 mM, 4fold activation of unprimed starch synthesis Zea mays
2.4.1.21 citrate 500 mM, 20fold activation of unprimed starch synthesis Zea mays
2.4.1.21 succinate 500 mM, 6fold activation of unprimed starch synthesis Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.21 additional information
-
 additional information amylopectin: 0.16 mg/ml, rabbit liver glycogen: 1.16 mg/ml Zea mays
2.4.1.21 0.033
-
 ADPglucose
-
 Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan Zea mays involved in starch biosynthesis ADP + alpha-1,4-polyglucan
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.21 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.21 ammonium sulfate, DEAE-cellulose, aminobutyl-Sepharose, ADP-hexanolamine-Sepharose, aminopropyl-Sepharose Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.21 seed
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.21 1.1
-
 unprimed reaction, synthase I Zea mays
2.4.1.21 2.3
-
 primed reaction, synthase I Zea mays

Storage Stability

EC Number Storage Stability Organism
2.4.1.21 -70°C, 50 mM Tris/acetate buffer, pH 7.5, 2.5 mM DTT, 10 mM EDTA, 5% w/v sucrose Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan citrate independent enzyme requires a glucan primer e.g. rabbit liver glycogen Zea mays ADP + alpha-1,4-polyglucan
-
 ?
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan involved in starch biosynthesis Zea mays ADP + alpha-1,4-polyglucan
-
 ?