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Literature summary extracted from

  • Denyer, K.; Smith, A.M.
    The purification and characterization of the two forms of soluble starch synthase from developing pea embryos (1992), Planta, 186, 609-617.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.21 additional information
-
additional information amylopectin: 0.19-0.79 mg/ml, value depends on isozyme and presence of citrate Pisum sativum
2.4.1.21 0.29
-
ADPglucose synthase I, absence of citrate Pisum sativum
2.4.1.21 0.48
-
ADPglucose synthase II, absence of citrate Pisum sativum
2.4.1.21 0.51
-
ADPglucose synthase II, presence of citrate Pisum sativum
2.4.1.21 0.97
-
ADPglucose synthase I, presence of citrate Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.21 soluble
-
Pisum sativum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.21 60000
-
x * 60000, synthase I, x * 77000, synthase II, SDS-PAGE Pisum sativum
2.4.1.21 77000
-
x * 60000, synthase I, x * 77000, synthase II, SDS-PAGE Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.21 Pisum sativum
-
pea
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.21 2 isoforms, DEAE-Sepharose, ammonium sulfate, Blue Sepharose, omega-aminobutyl agarose, Mono Q Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.21 seedling
-
Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.21 15.87
-
-
Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan no activity with UDPglucose Pisum sativum ADP + alpha-1,4-polyglucan
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.21 ? x * 60000, synthase I, x * 77000, synthase II, SDS-PAGE Pisum sativum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.21 15
-
stable at Pisum sativum
2.4.1.21 25
-
stable at Pisum sativum
2.4.1.21 35
-
inactivation Pisum sativum