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Literature summary extracted from

  • Rashid, N.; Cornista, J.; Ezaki, S.; Fukui, T.; Atomi, H.; Imanaka, T.
    Characterization of an archaeal cyclodextrin glucanotransferase with a novel C-terminal domain (2002), J. Bacteriol., 184, 777-784.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19
-
 Niallia circulans
2.4.1.19
-
 Thermoanaerobacterium thermosulfurigenes
2.4.1.19
-
 Paenibacillus macerans
2.4.1.19
-
 Geobacillus stearothermophilus
2.4.1.19
-
 Thermococcus sp.
2.4.1.19
-
 Klebsiella oxytoca
2.4.1.19 pET-8c vector, gene cloned and expressed in Escherichia coli BL21(DE3) Thermococcus kodakarensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.19
-
 Niallia circulans
2.4.1.19
-
 Thermoanaerobacterium thermosulfurigenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.19 Ca2+
-
 Bacillus sp. (in: Bacteria)
2.4.1.19 Ca2+
-
 Niallia circulans
2.4.1.19 Ca2+ thermal stability is remarkably increased by addition of calcium ions Geobacillus stearothermophilus
2.4.1.19 Ca2+ thermal stability is remarkably increased by addition of calcium ions Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.19 77000
-
 SDS-PAGE Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Bacillus sp. (in: Bacteria)
-
 1011
-
2.4.1.19 Bacillus sp. (in: Bacteria) 1011
-
 1011
-
2.4.1.19 Geobacillus stearothermophilus
-
-
-
2.4.1.19 Geobacillus stearothermophilus P31797
-
-
2.4.1.19 Klebsiella oxytoca P08704
-
-
2.4.1.19 Niallia circulans
-
-
-
2.4.1.19 Niallia circulans
-
 B. sp. var. alkalophilus No. 38-2, ATCC 21783
-
2.4.1.19 Niallia circulans P43379
-
-
2.4.1.19 Niallia circulans 251
-
-
-
2.4.1.19 Niallia circulans 251 P43379
-
-
2.4.1.19 Paenibacillus macerans P31835
-
-
2.4.1.19 Thermoanaerobacterium thermosulfurigenes
-
 EM1
-
2.4.1.19 Thermoanaerobacterium thermosulfurigenes EM1
-
 EM1
-
2.4.1.19 Thermococcus kodakarensis Q8X268 KOD1, UniProt accession No. Q8X268
-
2.4.1.19 Thermococcus sp.
-
 B1001
-
2.4.1.19 Thermococcus sp. Q9UWN2 B1001
-
2.4.1.19 Thermococcus sp. B1001
-
 B1001
-
2.4.1.19 Thermococcus sp. B1001 Q9UWN2 B1001
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19 recombinant enzyme Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 glycogen + H2O
-
 Thermococcus kodakarensis cyclodextrins
-
 ?
2.4.1.19 additional information
-
 Geobacillus stearothermophilus ?
-
 ?
2.4.1.19 additional information
-
 Niallia circulans ?
-
 ?
2.4.1.19 additional information pullulan is no substrate Thermococcus kodakarensis ?
-
 ?
2.4.1.19 additional information
-
 Niallia circulans 251 ?
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 Geobacillus stearothermophilus alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Niallia circulans alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Thermococcus sp. alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Thermococcus kodakarensis alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) 1011 alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Thermococcus sp. B1001 alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Niallia circulans 251 alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) beta-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Niallia circulans beta-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Thermococcus kodakarensis beta-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) 1011 beta-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Niallia circulans 251 beta-cyclodextrin
-
 r
2.4.1.19 soluble starch + H2O
-
 Niallia circulans cyclodextrins
-
 ?
2.4.1.19 soluble starch + H2O
-
 Thermococcus kodakarensis cyclodextrins
-
 ?
2.4.1.19 soluble starch + H2O
-
 Niallia circulans 251 cyclodextrins
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 CGT_TK
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 75
-
-
 Geobacillus stearothermophilus
2.4.1.19 75
-
 recombinant enzyme in absence of Ca2+ Thermococcus kodakarensis
2.4.1.19 80
-
-
 Geobacillus stearothermophilus
2.4.1.19 80
-
-
 Thermococcus kodakarensis
2.4.1.19 85 90 in presence of Ca2+ Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.19 85 100 extreme thermostability with addition of Ca2+, no loss of activity after 80 min at 85°C, half-life of 20 min at 100°C, recombinant enzyme half-life of 40 min at 100°C Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 5.5 6
-
 Thermococcus kodakarensis