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Literature summary extracted from

  • Lee, S.H.; Kim, Y.W.; Lee, S.; Auh, J.H.; Yoo, S.S.; Kim, T.J.; Kim, J.W.; Kim, S.T.; Rho, H.J.; Choi, J.H.; Kim, Y.B.; Park, K.H.
    Modulation of cyclizing activity and thermostability of cyclodextrin glucanotransferase and its application as an antistaling enzyme (2002), J. Agric. Food Chem., 50, 1411-1415.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.19 nutrition
-
Bacillus licheniformis
2.4.1.19 nutrition
-
Paenibacillus macerans
2.4.1.19 nutrition application as antistaling agent, retards the deterioration process in bread Geobacillus stearothermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19 subcloned on pUB140, expressed in Bacillus subtilis LKS88 Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.19 66800
-
wild-type and mutants, SDS-PAGE Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Bacillus licheniformis
-
-
-
2.4.1.19 Geobacillus stearothermophilus
-
ET1
-
2.4.1.19 Geobacillus stearothermophilus
-
NO2
-
2.4.1.19 Geobacillus stearothermophilus ET1
-
ET1
-
2.4.1.19 Geobacillus stearothermophilus NO2
-
NO2
-
2.4.1.19 Klebsiella pneumoniae
-
-
-
2.4.1.19 Niallia circulans P43379
-
-
2.4.1.19 Niallia circulans 251 P43379
-
-
2.4.1.19 Paenibacillus macerans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19 wild-type and mutant enzymes Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 alpha-cyclodextrin + glycosyl acceptor
-
Geobacillus stearothermophilus beta-cyclodextrin + maltooligosaccharide
-
r
2.4.1.19 alpha-cyclodextrin + glycosyl acceptor
-
Paenibacillus macerans beta-cyclodextrin + maltooligosaccharide
-
r
2.4.1.19 alpha-cyclodextrin + glycosyl acceptor
-
Geobacillus stearothermophilus NO2 beta-cyclodextrin + maltooligosaccharide
-
r
2.4.1.19 alpha-cyclodextrin + glycosyl acceptor
-
Geobacillus stearothermophilus ET1 beta-cyclodextrin + maltooligosaccharide
-
r
2.4.1.19 soluble starch + glycosyl acceptor
-
Geobacillus stearothermophilus maltose + maltotriose + maltotetraose + maltopentaose
-
r
2.4.1.19 soluble starch + glycosyl acceptor
-
Geobacillus stearothermophilus NO2 maltose + maltotriose + maltotetraose + maltopentaose
-
r
2.4.1.19 soluble starch + glycosyl acceptor
-
Geobacillus stearothermophilus ET1 maltose + maltotriose + maltotetraose + maltopentaose
-
r

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 CGTse ET1
-
Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 60
-
-
Paenibacillus macerans
2.4.1.19 60
-
mutant strain F191G Geobacillus stearothermophilus
2.4.1.19 75
-
mutant strain F251I Geobacillus stearothermophilus
2.4.1.19 80
-
strain ET1 Geobacillus stearothermophilus