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Literature summary extracted from

  • Fujiwara, S.; Kakihara, H.; Sakaguchi, K.; Imanaka, T.
    Analysis of mutations in cyclodextrin glucanotransferase from Bacillus stearothermophilus which affect cyclization characteristics and thermostability (1992), J. Bacteriol., 174, 7478-7481.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19
-
Paenibacillus macerans
2.4.1.19 strain NO2, mutant CGTases constructed from cgt1 gene and expressed in Bacillus subtilis NA-1 Geobacillus stearothermophilus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.19 F191Y Phe at position 191 replaced by Tyr Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Geobacillus stearothermophilus
-
NO2
-
2.4.1.19 Geobacillus stearothermophilus
-
TC-91
-
2.4.1.19 Geobacillus stearothermophilus NO2
-
NO2
-
2.4.1.19 Geobacillus stearothermophilus TC-91
-
TC-91
-
2.4.1.19 Klebsiella oxytoca
-
formerly Klebsiella pneumoniae M5a1
-
2.4.1.19 Niallia circulans
-
no. 8
-
2.4.1.19 Niallia circulans 8
-
no. 8
-
2.4.1.19 Paenibacillus macerans
-
IFO3490 (NRRL B-388)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19 recombinant enzymes, partially Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 amylose + glycosyl acceptor
-
Niallia circulans ?
-
r
2.4.1.19 amylose + glycosyl acceptor NO2, spiral amylose Geobacillus stearothermophilus ?
-
r
2.4.1.19 amylose + glycosyl acceptor NO2, spiral amylose Geobacillus stearothermophilus NO2 ?
-
r
2.4.1.19 amylose + glycosyl acceptor NO2, spiral amylose Geobacillus stearothermophilus TC-91 ?
-
r
2.4.1.19 amylose + glycosyl acceptor
-
Niallia circulans 8 ?
-
r
2.4.1.19 soluble starch + glycosyl acceptor
-
Geobacillus stearothermophilus cyclodextrins
-
r
2.4.1.19 soluble starch + glycosyl acceptor
-
Niallia circulans cyclodextrins
-
r
2.4.1.19 soluble starch + glycosyl acceptor
-
Paenibacillus macerans cyclodextrins
-
r
2.4.1.19 soluble starch + glycosyl acceptor
-
Klebsiella oxytoca cyclodextrins
-
r
2.4.1.19 soluble starch + glycosyl acceptor
-
Geobacillus stearothermophilus NO2 cyclodextrins
-
r
2.4.1.19 soluble starch + glycosyl acceptor
-
Geobacillus stearothermophilus TC-91 cyclodextrins
-
r
2.4.1.19 soluble starch + glycosyl acceptor
-
Niallia circulans 8 cyclodextrins
-
r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 55
-
-
Niallia circulans
2.4.1.19 55
-
-
Paenibacillus macerans
2.4.1.19 55
-
NO2, mutant enzymes Geobacillus stearothermophilus
2.4.1.19 65
-
strain NO2 Geobacillus stearothermophilus