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Literature summary extracted from

  • Bender, H.
    Cyclodextrin glucanotransferase from Klebsiella pneumoniae. 1. Formation, purification and properties of the enzyme from Klebsiella pneumoniae M 5 al (1977), Arch. Microbiol., 111, 271-282.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.19 extracellular
-
 Paenibacillus macerans
-
-
2.4.1.19 extracellular
-
 Klebsiella oxytoca
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Klebsiella oxytoca
-
 formerly Klebsiella pneumoniae M5a1
-
2.4.1.19 Klebsiella pneumoniae
-
 M5
-
2.4.1.19 Klebsiella pneumoniae M5
-
 M5
-
2.4.1.19 Paenibacillus macerans
-
-
-
2.4.1.19 Priestia megaterium
-
 No5
-
2.4.1.19 Priestia megaterium No5
-
 No5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 alpha-1,4-glucan + glycosyl acceptor
-
 Priestia megaterium cyclohexaamylose + cycloheptaamylose + cyclooctaamylose product ratio 1: 2.4: 1 r
2.4.1.19 alpha-1,4-glucan + glycosyl acceptor
-
 Priestia megaterium No5 cyclohexaamylose + cycloheptaamylose + cyclooctaamylose product ratio 1: 2.4: 1 r
2.4.1.19 amylopectin + glycosyl acceptor
-
 Klebsiella oxytoca cycloheptaamylose + cyclohexaamylose + exo-branched cyclohexaamylose
-
 r
2.4.1.19 cycloheptaamylose + glycosyl acceptor
-
 Klebsiella oxytoca ?
-
 r
2.4.1.19 cyclohexaamylose + glycosyl acceptor
-
 Klebsiella oxytoca ?
-
 r
2.4.1.19 soluble starch + H2O potato starch Klebsiella pneumoniae cyclodextrins
-
 ?
2.4.1.19 soluble starch + H2O potato starch Paenibacillus macerans cyclodextrins
-
 ?
2.4.1.19 soluble starch + H2O potato starch Klebsiella pneumoniae M5 cyclodextrins
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.19 45 55 stable up to 45°C, 13.4% activity at 50°C, no activity above 55°C Klebsiella oxytoca
2.4.1.19 80
-
 15 min at 80°C, after incubation for 12 h reactivation to a little restactivity Klebsiella oxytoca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 5.2
-
 strain M5 a1 Klebsiella oxytoca