BRENDA - Enzyme Database show

Free fatty acids regulate two galactosyltransferases in chloroplast envelope membranes isolated from spinach leaves

Sakaki, T.; Kondo, N.; Yamada, M.; Plant Physiol. 94, 781-787 (1990)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.184
alpha-linolenic acid
0.3 mM, 28fold stimulation in the presence of 2 mM MgCl2, MgCl2 can be replaced by MnCl2, CaCl2 or high concetrations of KCl
Spinacia oleracea
2.4.1.184
hexadecatrienoic acid
approx. 3fold activation
Spinacia oleracea
2.4.1.184
linoleic acid
approx. 4fold activiation
Spinacia oleracea
2.4.1.184
oleic acid
approx. 4fold activiation
Spinacia oleracea
2.4.1.184
palmitic acid
approx. 2fold activiation
Spinacia oleracea
2.4.1.184
palmitoleic acid
approx. 4fold activiation
Spinacia oleracea
2.4.1.184
stearic acid
approx. 2fold activiation
Spinacia oleracea
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.184
chloroplast envelope
envelope
Spinacia oleracea
9941
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.184
Ca2+
alpha-linolenic acid stimulates activity in presence of CaCl2
Spinacia oleracea
2.4.1.184
K+
alpha-linolenic acid stimulates in presence of high concentrations of KCl
Spinacia oleracea
2.4.1.184
Mg2+
alpha-linolenic acid causes drastic increase in activity under limiting concentrations of MgCl2, without affecting its maximum activity at higher MgCl2 concentration, free alpha-linolenic acid alone does not affect the activity; EDTA inhibits stimulation; unsaturated 16- and 18-carbon fatty acids stimulate in presence of MgCl2
Spinacia oleracea
2.4.1.184
Mn2+
alpha-linolenic acid stimulates activity in presence of MnCl2
Spinacia oleracea
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.184
Spinacia oleracea
-
cv. New Asia
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.184
leaf
-
Spinacia oleracea
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.184
1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol
-
488815
Spinacia oleracea
1,2-diacyl-3-O-[alpha-D-galactosyl-(1->6)-beta-D-galactosyl]-sn-glycerol + 1,2-diacyl-sn-glycerol
-
488815
Spinacia oleracea
-
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.184
alpha-linolenic acid
0.3 mM, 28fold stimulation in the presence of 2 mM MgCl2, MgCl2 can be replaced by MnCl2, CaCl2 or high concetrations of KCl
Spinacia oleracea
2.4.1.184
hexadecatrienoic acid
approx. 3fold activation
Spinacia oleracea
2.4.1.184
linoleic acid
approx. 4fold activiation
Spinacia oleracea
2.4.1.184
oleic acid
approx. 4fold activiation
Spinacia oleracea
2.4.1.184
palmitic acid
approx. 2fold activiation
Spinacia oleracea
2.4.1.184
palmitoleic acid
approx. 4fold activiation
Spinacia oleracea
2.4.1.184
stearic acid
approx. 2fold activiation
Spinacia oleracea
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.184
chloroplast envelope
envelope
Spinacia oleracea
9941
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.184
Ca2+
alpha-linolenic acid stimulates activity in presence of CaCl2
Spinacia oleracea
2.4.1.184
K+
alpha-linolenic acid stimulates in presence of high concentrations of KCl
Spinacia oleracea
2.4.1.184
Mg2+
alpha-linolenic acid causes drastic increase in activity under limiting concentrations of MgCl2, without affecting its maximum activity at higher MgCl2 concentration, free alpha-linolenic acid alone does not affect the activity; EDTA inhibits stimulation; unsaturated 16- and 18-carbon fatty acids stimulate in presence of MgCl2
Spinacia oleracea
2.4.1.184
Mn2+
alpha-linolenic acid stimulates activity in presence of MnCl2
Spinacia oleracea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.184
leaf
-
Spinacia oleracea
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.184
1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol
-
488815
Spinacia oleracea
1,2-diacyl-3-O-[alpha-D-galactosyl-(1->6)-beta-D-galactosyl]-sn-glycerol + 1,2-diacyl-sn-glycerol
-
488815
Spinacia oleracea
-