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Literature summary extracted from

  • Kurkela, M.; Garcia-Horsman, J.A.; Luukkanen, L.; Moersky, S.; Taskinen, J.; Baumann, M.; Kostiainen, R.; Hirvonen, J.; Finel, M.
    Expression and Characterization of Recombinant Human UDP-glucuronosyltransferases (UGTs) (2003), J. Biol. Chem., 278, 3536-3544.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.17 Phospholipids
-
 Homo sapiens
2.4.1.17 Triton X-100 stimulates UGT1A9 activity Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.17 8 different UGT's expressed in baculovirus-infected cells as His-tagged proteins Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.17 Triton X-100 inhibition of UGT2B and almost all of the UGT1A isozymes, not inhibit glucuronidation of 1-naphthol nor scopoletin by UGT1A9, inhibition of entacapone glucuronidation tested on isozymes of UGT1A and UGT1B families, only for UGT1A9 showed reversible inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.17 0.061
-
 scopoletin apparent Km for membrane-bound UGT1AB Homo sapiens
2.4.1.17 0.229
-
 UDP-glucuronic acid apparent Km for membrane-bound UGT1AB Homo sapiens
2.4.1.17 0.704
-
 UDP-glucuronic acid apparent Km for purified UGT1AB Homo sapiens
2.4.1.17 0.79
-
 scopoletin apparent Km for purified UGT1AB Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.17 endoplasmic reticulum
-
 Homo sapiens 5783
-
2.4.1.17 microsome
-
 Homo sapiens
-
-
2.4.1.17 nuclear membrane
-
 Homo sapiens 31965
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.17 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.17 His-tagged UGT1A9 purified by immobilized metal-chelating chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.17 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.17 1-naphthol + UDP-glucuronate
-
 Homo sapiens naphth-1-yl-beta-D-glucuronide + UDP
-
 ?
2.4.1.17 scopoletin + UDPglucuronate
-
 Homo sapiens UDP + scopoletin O-glucuronide
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.17 ? suggested homodimer Homo sapiens
2.4.1.17 ? all values determined by SDS-PAGE Homo sapiens