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Literature summary extracted from

  • Iwano, H.; Yokota, H.; Ohgiya, S.; Yuasa, A.
    The significance of amino acid residue Asp446 for enzymic stability of rat UDP-glucuronosyltransferase UGT1A6 (1999), Arch. Biochem. Biophys., 363, 116-120.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.17 expressed in yeast AH22 cells Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.17 D446A UGT1A6 activity retained for 1-naphthol nor UDP-glucuronic acid Rattus norvegicus
2.4.1.17 D446E UGT1A6 activity retained for 1-naphthol nor UDP-glucuronic acid Rattus norvegicus
2.4.1.17 D446G UGT1A6 activity not detected for 1-naphthol nor UDP-glucuronic acid Rattus norvegicus
2.4.1.17 D446K UGT1A6 activity not detected for 1-naphthol nor UDP-glucuronic acid Rattus norvegicus
2.4.1.17 D446N UGT1A6 activity retained for 1-naphthol nor UDP-glucuronic acid Rattus norvegicus
2.4.1.17 D446q UGT1A6 activity retained for 1-naphthol nor UDP-glucuronic acid Rattus norvegicus
2.4.1.17 D446T UGT1A6 activity retained for 1-naphthol nor UDP-glucuronic acid Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.17 0.00246
-
 1-naphthol apparent Km for UGT1A6, 3mM UDP-glucuronic acid, mutants that retained activity shows similar Km values Rattus norvegicus
2.4.1.17 0.26
-
 UDP-glucuronic acid apparent Km for UGT1A6, 50mM 1-naphthol, mutants that retained activity shows similar Km values Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.17 endoplasmic reticulum
-
 Homo sapiens 5783
-
2.4.1.17 endoplasmic reticulum
-
 Rattus norvegicus 5783
-
2.4.1.17 microsome
-
 Homo sapiens
-
-
2.4.1.17 microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.17 54000
-
 UGT1A6 wild-type and all its mutants Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.17 Homo sapiens
-
-
-
2.4.1.17 Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.17 1-naphthol + UDP-glucuronate
-
 Homo sapiens naphth-1-yl-beta-D-glucuronide + UDP
-
 ?
2.4.1.17 1-naphthol + UDP-glucuronate
-
 Rattus norvegicus naphth-1-yl-beta-D-glucuronide + UDP
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.17 37
-
 wild type UGT1A6 stable for 30 min, mutants inactives after preincubation Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.17 additional information
-
 UGT1A6 mutants activity reduced at acidic pH Rattus norvegicus