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Literature summary extracted from

  • Schumacher, R.; Rao, G.S.; Rao, L.M.; Breuer, H.
    Steroidglucuronyltransferases. 3. Oestradiol-17 3-glucuronyltransferase of the mitochondria of pig intestine (1972), Hoppe-Seyler's Z. Physiol. Chem., 353, 1784-1788.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.17 cysteine 10 mM, slight activation Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.17 ATP inhibitor at 10 mM, no effect below Sus scrofa
2.4.1.17 diclofenac
-
 Sus scrofa
2.4.1.17 estrone competitive to estradiol-17beta Sus scrofa
2.4.1.17 UDP 0.05 mM inhibits 60% of the activity of the mitochondrial enzyme Sus scrofa
2.4.1.17 Zn2+ inhibitor above 10mM Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.17 0.045
-
 17beta-estradiol
-
 Sus scrofa
2.4.1.17 0.046
-
 estradiol-17beta range between 0.045 mM and 0.047 mM Sus scrofa
2.4.1.17 0.16
-
 UDPglucuronic acid
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.17 cytosol
-
 Sus scrofa 5829
-
2.4.1.17 microsome
-
 Sus scrofa
-
-
2.4.1.17 mitochondrion
-
 Sus scrofa 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.17 Ca2+ 20 mM, activation Sus scrofa
2.4.1.17 Mg2+ activation at 20 mM Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.17 Sus scrofa
-
 pig
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.17 small intestine
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.17 estradiol-17beta + UDPglucuronate
-
 Sus scrofa estradiol-17beta-3-monoglucuronide + UDP
-
 ?
2.4.1.17 estriol + UDPglucuronate
-
 Sus scrofa UDP + estriol-16 alpha-glucuronide
-
 ?
2.4.1.17 estrone + UDPglucuronate
-
 Sus scrofa UDP + estrone 3-O-glucuronide
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.17 42
-
 activity increases with rise in incubation temperature Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.17 40
-
 inactivation in absence of substrates Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.17 7.5
-
 Tris/HCl buffer, in Tris/maleate and phosphate buffers the enzyme shows this pH optimum but lower activity Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.17 0.008
-
 diclofenac
-
 Sus scrofa