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Literature summary extracted from

  • Rao, M.L.; Rao, G.S.; Breuer, H.
    Investigations on the kinetic properties of estrone glucuronyltransferase from pig kidney (1976), Biochim. Biophys. Acta, 452, 89-100.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.17 ATP
-
 Sus scrofa
2.4.1.17 bilirubin linear noncompetitive inhibition towards estrone Sus scrofa
2.4.1.17 Cd2+ complete inactivation at 10 mM Sus scrofa
2.4.1.17 Cu2+ 10 mM, complete inactivation Sus scrofa
2.4.1.17 EDTA not inhibited Sus scrofa
2.4.1.17 estradiol 17alpha or 17beta, noncompetitive inhibition towards estrone Sus scrofa
2.4.1.17 estriol linear noncompetitive inhibition towards estrone Sus scrofa
2.4.1.17 Hg2+ complete inactivation Sus scrofa
2.4.1.17 additional information dithioerythritol; not inhibitory: N-ethylmaleimide; product inhibition Sus scrofa
2.4.1.17 p-chloromercuribenzoate
-
 Sus scrofa
2.4.1.17 Phenolphthalein linear noncompetitive inhibition towards estrone Sus scrofa
2.4.1.17 testosterone linear noncompetitive inhibition towards estrone Sus scrofa
2.4.1.17 UDP noncompetitive inhibition towards UDPglucuronic acid at saturating concentration of UDPglucuronic acid, linear and uncompetitive inhibition with saturating concentration of estrone and varying concentration of UDPglucuronic acid Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.17 0.0035
-
 estrone
-
 Sus scrofa
2.4.1.17 0.025
-
 UDPglucuronic acid
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.17 microsome
-
 Sus scrofa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.17 Ca2+ 10 mM, 30% activation Sus scrofa
2.4.1.17 Mg2+ 10 mM, 30% activation Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.17 Sus scrofa
-
 pig
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.17 kidney
-
 Sus scrofa
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.17 -20°C, microsomal preparation, more than 4 months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.17 estrone + UDPglucuronate affinity increases during storage period at -20ºC for more than 4 months Sus scrofa UDP + estrone 3-O-glucuronide
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.17 55
-
-
 Sus scrofa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.17 2 45 linear estrone glucuronide formation Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.17 30
-
 15 min, absence of substrates, slight inactivation Sus scrofa
2.4.1.17 48
-
 15 min, 50% loss of activity Sus scrofa
2.4.1.17 70
-
 15 min, complete inactivation Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.17 7.3 8.7 estrone Sus scrofa