Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Burchell, B.; Weatherill, P.
    4-Nitrophenol UDPglucuronyltransferase (rat liver) (1981), Methods Enzymol., 77, 169-177.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.17 Alkyl ketones activation Rattus norvegicus
2.4.1.17 Diethylnitrosamine 2fold to 3fold activation towards 2-aminophenol Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.17 Lubrol 12A9
-
 Rattus norvegicus
2.4.1.17 additional information not inhibitory: N-ethylmaleimide Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.17 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.17 Mg2+ 2 to 20 mM, 30% increased activity Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.17 57000
-
 x * 57000, SDS-polyacrylamide gel electrophoresis Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.17 Rattus norvegicus
-
 Wistar strain
-
2.4.1.17 Rattus norvegicus Wistar
-
 Wistar strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.17 several methods including chromatofocusing, combined with anion exchange chromatography and/or affinity chromatography of UDP-hexanolamine Sepharose Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.17 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.17 additional information
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.4.1.17 -20°C, 20% w/v glycerol, complete loss of activity Rattus norvegicus
2.4.1.17 0°C, half-life 18-20 days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.17 1-naphthol + UDP-glucuronate
-
 Rattus norvegicus naphth-1-yl-beta-D-glucuronide + UDP
-
 ?
2.4.1.17 1-naphthol + UDP-glucuronate
-
 Rattus norvegicus Wistar naphth-1-yl-beta-D-glucuronide + UDP
-
 ?
2.4.1.17 4-nitrophenol + UDP-glucuronate
-
 Rattus norvegicus 4-nitrophenyl beta-D-glucuronide + UDP
-
 ?
2.4.1.17 4-nitrophenol + UDP-glucuronate
-
 Rattus norvegicus Wistar 4-nitrophenyl beta-D-glucuronide + UDP
-
 ?
2.4.1.17 morphine + UDP-glucuronate
-
 Rattus norvegicus UDP + morphine 3,6-diglucuronide
-
 ?
2.4.1.17 morphine + UDP-glucuronate
-
 Rattus norvegicus Wistar UDP + morphine 3,6-diglucuronide
-
 ?
2.4.1.17 testosterone + UDPglucuronate
-
 Rattus norvegicus UDP + testosterone O-glucuronide
-
 ?
2.4.1.17 testosterone + UDPglucuronate
-
 Rattus norvegicus Wistar UDP + testosterone O-glucuronide
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.17 ? x * 57000, SDS-polyacrylamide gel electrophoresis Rattus norvegicus
2.4.1.17 ? additional values for rat isozymes ranging from 51000 to 59000 Rattus norvegicus
2.4.1.17 ? all values determined by SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.17 37
-
 inactivation in 15 min Rattus norvegicus