Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Falany, C.N.; Tephly, T.R.
    Separation, purification and characterization of three isoenzymes of UDP-glucuronyltransferase from rat liver microsomes (1983), Arch. Biochem. Biophys., 227, 248-258.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.17 3-methylcholanthrene marked induction of hepatic microsomal UDPGT activity, sevenfold increase in the rate of 4-methylumbelliferone glucuronidation, more than 3fold increase towards 4-nitrophenol and fourfold increase towards 1-naphthol Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.17 microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.17 52000
-
 3-OH androgenic UDP-glucuronyltransferase, chromatofocusing and affinity chromatography Rattus norvegicus
2.4.1.17 56000
-
 3-methylcholanthrene-inducible 4-nitrophenol UDP-glucuronyltransferase, chromatofocusing and affinity chromatography Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.17 Rattus norvegicus
-
 Sprague-Dawley rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.17 chromatofocusing and affinity chromatography on UDP-hexanolamine sepharose, three isoenzymes Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.17 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.17 1-naphthol + UDP-glucuronate
-
 Rattus norvegicus naphth-1-yl-beta-D-glucuronide + UDP
-
 ?
2.4.1.17 4-methylumbelliferone + UDP-glucuronate
-
 Rattus norvegicus UDP + 4-methylumbelliferyl-7-O-beta-D-glucuronide
-
 ?
2.4.1.17 4-nitrophenol + UDP-glucuronate 3-methylcholanthrene-inducible 4-nitrophenol UDP-glucuronyltransferase and 17-OH steroid UDP-glucuronyltransferase isoenzymes Rattus norvegicus 4-nitrophenyl beta-D-glucuronide + UDP
-
 ?
2.4.1.17 androsterone + UDPglucuronate
-
 Rattus norvegicus UDP + androsterone O-glucuronide
-
 ?
2.4.1.17 beta-estradiol + UDPglucuronate
-
 Rattus norvegicus beta-estradiol-3-glucuronide + UDP
-
 ?
2.4.1.17 etiocholanone + UDPglucuronate 3-OH androgenic UDP-glucuronyltransferase isoenzyme Rattus norvegicus UDP + etiocholanone O-glucuronide
-
 ?
2.4.1.17 testosterone + UDPglucuronate isozyme 17-OH steroid UDPGT Rattus norvegicus UDP + testosterone O-glucuronide
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.17 ? 4-nitrophenol UDP-glucuronyltransferase Rattus norvegicus
2.4.1.17 ? all values determined by SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.17 -60
-
 as pellets with a 1.15% KCl overlay, no apparent loss of activity for three months Rattus norvegicus