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Literature summary extracted from

  • Hayashi, T.; Matsuda, K.
    Biosynthesis of xyloglucan in suspension-cultured soybean cells. Occurrence and some properties of xyloglucan 4-beta-D-glucosyltransferase and 6-alpha-D-xylosyltransferase (1981), J. Biol. Chem., 256, 11117-11122.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.168 UDP-xylose activation, 0.01-0.03 mM Glycine max
2.4.2.39 GDP-D-glucose activation Glycine max
2.4.2.39 GDP-D-glucose about half as effective as UDPglucose, in concentrations exceeding UDPxylose Glycine max
2.4.2.39 TDPglucose activation, as effective as UDPglucose, in concentrations exceeding UDPxylose Glycine max
2.4.2.39 UDPglucose activation, xylose is effectively incorporated in the presence of UDPglucose, the transfer must be preceded by elongation of the beta-1,4-glucan-backbone, because xylosyl residues constitute the side chains, in concentrations exceeding UDPxylose, no activation by CDPglucose or ADPglucose Glycine max

General Stability

EC Number General Stability Organism
2.4.1.168 DTT, 1 mM, EDTA, 1 mM, sucrose, 0.4 M, bovine serum albumin, 0.1%, stabilize Glycine max
2.4.2.39 DTT, 1 mM, EDTA, 1 mM, sucrose, 0.4 M, bovine serum albumin, 0.1%, stabilize Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.168 GTPglucose weak Glycine max
2.4.1.168 additional information no inhibition by tunicamycin, ATP or GTP Glycine max
2.4.1.168 TDPglucose weak Glycine max
2.4.1.168 UTP
-
 Glycine max
2.4.2.39 Detergents no solubilization possible due to this inhibition Glycine max
2.4.2.39 EDTA weak Glycine max
2.4.2.39 additional information no inhibition by tunicamycin, ATP or GTP Glycine max
2.4.2.39 phosphate buffer
-
 Glycine max
2.4.2.39 Tris-HCl buffer
-
 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.39 additional information
-
 additional information kinetic study Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.168 membrane
-
 Glycine max 16020
-
2.4.2.39 membrane
-
 Glycine max 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.39 Mg2+ activation, can replace Mn2+ to some extent Glycine max
2.4.2.39 Mn2+ activation Glycine max
2.4.2.39 Mn2+ 10 mM Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.168 UDP-D-glucose + xyloglucan Glycine max responsible for the formation of the xyloglucan backbone UDP + glucosylxyloglucan
-
 ?
2.4.2.39 UDP-D-xylose + xyloglucan Glycine max responsible for xyloglucan side-chain formation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.168 Glycine max
-
 soy bean
-
2.4.2.39 Glycine max
-
 soy bean
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.168 cell suspension culture
-
 Glycine max
-
2.4.2.39 cell suspension culture
-
 Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.168 UDP-D-glucose + (glucosyl)xyloglucan no acceptor: cello-oligosaccharides and fragment oligosaccharides from endoglucanase digest Glycine max UDP + (glucosyl-glucosyl)xyloglucan forms a beta-1,4-D-glucosyl-D-glucose linkage ?
2.4.1.168 UDP-D-glucose + (glucosyl)xyloglucan transfers a beta-D-glucosyl residue from UDPglucose on to a glucose residue in xyloglucan, other glucosyl-acceptors are beta-1,3-glucan and xylan Glycine max UDP + (glucosyl-glucosyl)xyloglucan forms a beta-1,4-D-glucosyl-D-glucose linkage ?
2.4.1.168 UDP-D-glucose + xyloglucan responsible for the formation of the xyloglucan backbone Glycine max UDP + glucosylxyloglucan
-
 ?
2.4.2.39 UDP-D-xylose + (glucosyl)xyloglucan no acceptors are cello-oligosaccharides and fragment oligosaccharides from endoglucanase digest Glycine max UDP + (xylosyl-glucosyl)xyloglucan forms an alpha-1,6-D-xylosyl-D-glucose linkage ?
2.4.2.39 UDP-D-xylose + (glucosyl)xyloglucan other xylosyl-acceptors are beta-1,3-glucan and xylan Glycine max UDP + (xylosyl-glucosyl)xyloglucan forms an alpha-1,6-D-xylosyl-D-glucose linkage ?
2.4.2.39 UDP-D-xylose + (glucosyl)xyloglucan transfers an alpha-D-xylosyl residue from UDP-D-xylose to a glucose residue in xyloglucan Glycine max UDP + (xylosyl-glucosyl)xyloglucan forms an alpha-1,6-D-xylosyl-D-glucose linkage ?
2.4.2.39 UDP-D-xylose + xyloglucan responsible for xyloglucan side-chain formation Glycine max ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.168 35
-
-
 Glycine max
2.4.2.39 35
-
-
 Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.168 6.5 7
-
 Glycine max
2.4.2.39 6
-
 incorporation of xylosyl residues into polymeric acceptors Glycine max
2.4.2.39 6.5 7 UDPglucose + UDPxylose Glycine max