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Literature summary extracted from

  • Ruiz-Herrera, J.; Lopez-Romero, E.; Bartnicki-Garcia, S.
    Properties of chitin synthetase in isolated chitosomes from yeast cells of Mucor rouxii (1977), J. Biol. Chem., 252, 3338-3343.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.16 GlcNAc stimulates Amylomyces rouxii

General Stability

EC Number General Stability Organism
2.4.1.16 the zymogen is highly stable Amylomyces rouxii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.16 ADP 6.25 mM Amylomyces rouxii
2.4.1.16 AMP 6.25 mM Amylomyces rouxii
2.4.1.16 ATP 6.25 mM Amylomyces rouxii
2.4.1.16 Ca2+ 5-10 mM Amylomyces rouxii
2.4.1.16 Co2+ 5-10 mM Amylomyces rouxii
2.4.1.16 Polyoxin D
-
 Amylomyces rouxii
2.4.1.16 UDP
-
 Amylomyces rouxii
2.4.1.16 UMP 6.25 mM Amylomyces rouxii
2.4.1.16 UTP 6.25 mM Amylomyces rouxii
2.4.1.16 Zn2+ 5-10 mM Amylomyces rouxii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.16 chitosome
-
 Amylomyces rouxii 45009
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.16 Co2+ optimal concentration is 5-20 mM Amylomyces rouxii
2.4.1.16 Mg2+ Mg2+ or Mn2+ required Amylomyces rouxii
2.4.1.16 Mn2+ Mn2+ or Mg2+ required Amylomyces rouxii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.16 700000
-
 gel filtration Amylomyces rouxii

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.16 Amylomyces rouxii
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.16 proteolytic modification enzyme exists in the zymogenic form and requires proteolytic activation Amylomyces rouxii

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.16
-
 Amylomyces rouxii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.16 0.01639
-
-
 Amylomyces rouxii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.16 UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n
-
 Amylomyces rouxii UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.16 40 50 5 min, 20-25% loss of activity Amylomyces rouxii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.16 6.5
-
-
 Amylomyces rouxii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.16 0.00065
-
 polyoxin
-
 Amylomyces rouxii
2.4.1.16 0.4
-
 UDP
-
 Amylomyces rouxii