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Literature summary extracted from

  • Nung Jan, Y.
    Properties and cellular localization of chitin synthetase in Phycomyces blakesleeanus (1974), J. Biol. Chem., 249, 1973-1979.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.16 ATP 0.25 mM activates by 33%, concentrations up to 0.5 mM stimulate Phycomyces blakesleeanus
2.4.1.16 GlcNAc stimulates Phycomyces blakesleeanus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.16 ATP above 1 mM Phycomyces blakesleeanus
2.4.1.16 c3',5'-AMP 2 mM, slight inhibition Phycomyces blakesleeanus
2.4.1.16 Polyoxin D
-
 Phycomyces blakesleeanus
2.4.1.16 UDP 0.5 mM, 28% inhibition Phycomyces blakesleeanus
2.4.1.16 UDPgalacturonic acid 1 mM, about 20% inhibition Phycomyces blakesleeanus
2.4.1.16 UDPglucose 1 mM, about 20% inhibition Phycomyces blakesleeanus
2.4.1.16 UDPglucuronic acid 1 mM, about 20% inhibition Phycomyces blakesleeanus
2.4.1.16 UDPmannose 1 mM, about 20% inhibition Phycomyces blakesleeanus
2.4.1.16 UDPxylose 1 mM, about 20% inhibition Phycomyces blakesleeanus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.16 0.6
-
 UDP-GlcNAc
-
 Phycomyces blakesleeanus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.16 plasma membrane
-
 Phycomyces blakesleeanus 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.16 Mg2+ stimulates Phycomyces blakesleeanus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.16 Phycomyces blakesleeanus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.16 mycelium
-
 Phycomyces blakesleeanus
-
2.4.1.16 sporangiospore
-
 Phycomyces blakesleeanus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.16 UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n
-
 Phycomyces blakesleeanus UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1 chitin ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.16 28
-
-
 Phycomyces blakesleeanus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.16 27 42 50% of maximal activity at 17°C and at 42°C Phycomyces blakesleeanus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.16 6.5
-
-
 Phycomyces blakesleeanus