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Literature summary extracted from

  • Gay, L.; Hebraud, M.; Girard, V.; Fevre, M.
    Chitin synthase activity from Neocallimastix frontalis, an anaerobic rumen fungus (1989), J. Gen. Microbiol., 135, 279-283.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.16 GlcNAc 20 mM stimulates, allosteric activator Neocallimastix frontalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.16 Polyoxin D
-
 Neocallimastix frontalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.16 2
-
 UDP-GlcNAc
-
 Neocallimastix frontalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.16 plasma membrane
-
 Neocallimastix frontalis 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.16 Mg2+ divalent cation required for maximal activity, Mg2+ is most efficient Neocallimastix frontalis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.16 Neocallimastix frontalis
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.16 proteolytic modification following treatment with trypsin, the chitin synthase activity is increased by 6fold, indicating that most of the chitin synthase activity is zymogenic Neocallimastix frontalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.16 mycelium
-
 Neocallimastix frontalis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.16 UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n
-
 Neocallimastix frontalis UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1 chitin ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.16 32
-
-
 Neocallimastix frontalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.16 8.5
-
 10 mM Tris/HCl buffer Neocallimastix frontalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.16 0.004
-
 Polyoxin D
-
 Neocallimastix frontalis