BRENDA - Enzyme Database

Formation of flavonol 3-O-diglycosides and flavonol 3-O-triglycosides by enzyme extracts from anthers of Tulipa cv apeldoorn - characterization and activity of 3 different O-glycosyltransferases during anther development

Kleinehollenhorst, G.; Behrens, H.; Pegels, G.; Strunk, N.; Wiermann, R.; Z. Naturforsch. C 37c, 587-599 (1982)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.91
2-mercaptoethanol
1 mM, activation to 120%
Tulipa hybrid cultivar
2.4.1.91
dithioerythritol
1 mM, activation to 124%
Tulipa hybrid cultivar
2.4.1.91
glutathione
1 mM, activation to 118%
Tulipa hybrid cultivar
2.4.1.91
sucrose
5%, activation to 123%
Tulipa hybrid cultivar
2.4.1.159
2-mercaptoethanol
slight stimulation
Tulipa hybrid cultivar
2.4.1.159
dithioerythritol
slight stimulation
Tulipa hybrid cultivar
2.4.1.159
glutathione
slight stimulation
Tulipa hybrid cultivar
2.4.1.159
additional information
no stimulation by sucrose or bovine serum albumin
Tulipa hybrid cultivar
2.4.2.35
2-mercaptoethanol
slight stimulation
Tulipa hybrid cultivar
2.4.2.35
dithioerythritol
slight stimulation
Tulipa hybrid cultivar
2.4.2.35
glutathione
slight stimulation
Tulipa hybrid cultivar
2.4.2.35
additional information
no stimulation by bovine serum albumin, not affected by anions like chloride and sulfate
Tulipa hybrid cultivar
2.4.2.35
NH4+
high activation
Tulipa hybrid cultivar
2.4.2.35
sucrose
slight stimulation
Tulipa hybrid cultivar
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.91
PCMB
-
Tulipa hybrid cultivar
2.4.1.159
p-chloromercuribenzoate
weak
Tulipa hybrid cultivar
2.4.2.35
Cu2+
high inhibition when applied in high concentrations
Tulipa hybrid cultivar
2.4.2.35
Mn2+
-
Tulipa hybrid cultivar
2.4.2.35
p-chloromercuribenzoate
-
Tulipa hybrid cultivar
2.4.2.35
Zn2+
high inhibition when applied in high concentrations
Tulipa hybrid cultivar
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.159
Mg2+
slight stimulation
Tulipa hybrid cultivar
2.4.1.159
additional information
no activation by Ca2+, NH4+, Mn2+, Cl- or SO42-
Tulipa hybrid cultivar
2.4.2.35
Ca2+
high activation
Tulipa hybrid cultivar
2.4.2.35
additional information
no effect by Mg2+
Tulipa hybrid cultivar
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.91
40000
-
gel filtration
Tulipa hybrid cultivar
2.4.1.159
40000
-
gel filtration
Tulipa hybrid cultivar
2.4.2.35
30000
-
gel filtration
Tulipa hybrid cultivar
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.4.1.159
UDPrhamnose + flavonol 3-O-glucoside
Tulipa hybrid cultivar
involved in flavonoid metabolism, pathway of flavonol 3-O-triglycoside biosynthesis
UDP + flavonol 3-O-rhamnosylglucoside
-
-
?
2.4.2.35
UDP-D-xylose + flavonol 3-O-glycoside
Tulipa hybrid cultivar
involved in flavonoid metabolism, pathway of flavonol 3-O-triglycoside biosynthesis
UDP + flavonol 3-O-D-xylosylglycoside
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.4.1.91
Tulipa hybrid cultivar
-
cv. Apeldoorn
-
2.4.1.159
Tulipa hybrid cultivar
-
tulip, cv. Apeldoorn
-
2.4.2.35
Tulipa hybrid cultivar
-
tulip, cv. Apeldoorn
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.4.1.91
partial
Tulipa hybrid cultivar
2.4.1.159
partial
Tulipa hybrid cultivar
2.4.2.35
partial by a method that includes Sephadex G-200 chromatography, Sephadex G-15 chromatography, isoelectric focusing and DEAE-Sephadex chromatography
Tulipa hybrid cultivar
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.91
anther
-
Tulipa hybrid cultivar
-
2.4.1.159
anther
-
Tulipa hybrid cultivar
-
2.4.2.35
anther
-
Tulipa hybrid cultivar
-
2.4.2.35
pollen
at the stage of middle postmeiotic pollen ripening
Tulipa hybrid cultivar
-
2.4.2.35
tapetum
-
Tulipa hybrid cultivar
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.4.1.91
UDP-alpha-D-glucose + isorhamnetin
isorhamnetin is the best acceptor
488625
Tulipa hybrid cultivar
UDP + isorhamnetin 3-O-beta-D-glucoside
-
-
-
r
2.4.1.91
UDPgalactose + isorhamnetin
isorhamnetin is the best acceptor, 45% of the activity compared to reaction with UDPglucose
488625
Tulipa hybrid cultivar
UDP + isorhamnetin 3-O-glucoside
-
-
-
r
2.4.1.91
UDPglucose + kaempferol
45% of the activity with isorhamnetin
488625
Tulipa hybrid cultivar
UDP + kaempferol 3-O-glucoside
-
-
-
r
2.4.1.91
UDPglucose + kaempferol-4'-O-methylether
13% of the activity with isorhamnetin
488625
Tulipa hybrid cultivar
UDP + ?
-
-
-
r
2.4.1.91
UDPglucose + quercetin
93% of the activity with isorhamnetin
488625
Tulipa hybrid cultivar
UDP + quercetin 3-O-glucoside
-
-
-
r
2.4.1.91
UDPglucose + rutin
4% of the activity with isorhamnetin
488625
Tulipa hybrid cultivar
UDP + rutin 3-O-glucoside
-
-
-
r
2.4.1.159
UDPrhamnose + flavonol 3-O-diglycoside
-
488625
Tulipa hybrid cultivar
UDP + flavonol 3-O-triglycoside
-
488625
Tulipa hybrid cultivar
?
2.4.1.159
UDPrhamnose + flavonol 3-O-glucoside
acceptor substrates are 3-O-glucosides of quercetin, isorhamnetin or kaempferol, rutin, less efficient acceptors: quercetin 3-O-galactoside or quercetin 3-O-rhamnoside, kaempferol 3-O-rhamnoside or myrecetin 3-O-rhamnoside, no substrates: aglycones
488625
Tulipa hybrid cultivar
UDP + flavonol 3-O-rhamnosylglucoside
i.e. 3-O-rutinosides
488625
Tulipa hybrid cultivar
?
2.4.1.159
UDPrhamnose + flavonol 3-O-glucoside
involved in flavonoid metabolism, pathway of flavonol 3-O-triglycoside biosynthesis
488625
Tulipa hybrid cultivar
UDP + flavonol 3-O-rhamnosylglucoside
-
-
-
?
2.4.2.35
additional information
aglycones are no substrates
488625
Tulipa hybrid cultivar
?
-
-
-
?
2.4.2.35
UDP-D-xylose + flavonol 3-O-diglycoside
-
488625
Tulipa hybrid cultivar
UDP + flavonol 3-O-triglycoside
-
-
-
?
2.4.2.35
UDP-D-xylose + flavonol 3-O-glycoside
-
488625
Tulipa hybrid cultivar
UDP + flavonol 3-O-D-xylosylglycoside
-
488625
Tulipa hybrid cultivar
?
2.4.2.35
UDP-D-xylose + flavonol 3-O-glycoside
involved in flavonoid metabolism, pathway of flavonol 3-O-triglycoside biosynthesis
488625
Tulipa hybrid cultivar
UDP + flavonol 3-O-D-xylosylglycoside
-
-
-
?
2.4.2.35
UDP-D-xylose + isorhamnetin 3,7-O-diglucoside
poor substrate
488625
Tulipa hybrid cultivar
?
-
-
-
?
2.4.2.35
UDP-D-xylose + isorhamnetin 3-O-glucoside
-
488625
Tulipa hybrid cultivar
UDP + isorhamnetin 3-O-xylosylglucoside
-
-
-
?
2.4.2.35
UDP-D-xylose + kaempferol 3-O-glucoside
-
488625
Tulipa hybrid cultivar
UDP + kaempferol 3-O-xylosylglucoside
-
-
-
?
2.4.2.35
UDP-D-xylose + kaempferol 3-O-rhamnoside
poor substrate
488625
Tulipa hybrid cultivar
UDP + kaempferol 3-O-xylosylrhamnoside
-
-
-
?
2.4.2.35
UDP-D-xylose + myricetin 3-O-rhamnoside
poor substrate
488625
Tulipa hybrid cultivar
UDP + myricetin 3-O-xylosylrhamnoside
-
-
-
?
2.4.2.35
UDP-D-xylose + quercetin 3-O-arabinoglucoside
poor substrate
488625
Tulipa hybrid cultivar
UDP + quercetin 3-O-xylosylarabinoglucoside
-
-
-
?
2.4.2.35
UDP-D-xylose + quercetin 3-O-galactoside
best substrate tested
488625
Tulipa hybrid cultivar
UDP + quercetin 3-O-xylosylgalactoside
-
-
-
?
2.4.2.35
UDP-D-xylose + quercetin 3-O-glucoside
-
488625
Tulipa hybrid cultivar
UDP + quercetin 3-O-xylosylglucoside
-
-
-
?
2.4.2.35
UDP-D-xylose + quercetin 3-O-glucoside
poor substrate
488625
Tulipa hybrid cultivar
UDP + quercetin 3-O-D-xylosylglucoside
-
-
-
?
2.4.2.35
UDP-D-xylose + quercetin 3-O-rhamnoside
poor substrate
488625
Tulipa hybrid cultivar
UDP + quercetin 3-O-xylosylrhamnoside
-
488625
Tulipa hybrid cultivar
?
2.4.2.35
UDP-D-xylose + quercetin 3-O-rhamnosylglucoside
i.e. rutin, poor substrate
488625
Tulipa hybrid cultivar
UDP + quercetin 3-O-D-xylosylrhamnosylglucoside
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.91
8.5
9
-
Tulipa hybrid cultivar
2.4.1.159
8.5
9
-
Tulipa hybrid cultivar
2.4.2.35
8.5
9
-
Tulipa hybrid cultivar
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.91
2-mercaptoethanol
1 mM, activation to 120%
Tulipa hybrid cultivar
2.4.1.91
dithioerythritol
1 mM, activation to 124%
Tulipa hybrid cultivar
2.4.1.91
glutathione
1 mM, activation to 118%
Tulipa hybrid cultivar
2.4.1.91
sucrose
5%, activation to 123%
Tulipa hybrid cultivar
2.4.1.159
2-mercaptoethanol
slight stimulation
Tulipa hybrid cultivar
2.4.1.159
dithioerythritol
slight stimulation
Tulipa hybrid cultivar
2.4.1.159
glutathione
slight stimulation
Tulipa hybrid cultivar
2.4.1.159
additional information
no stimulation by sucrose or bovine serum albumin
Tulipa hybrid cultivar
2.4.2.35
2-mercaptoethanol
slight stimulation
Tulipa hybrid cultivar
2.4.2.35
dithioerythritol
slight stimulation
Tulipa hybrid cultivar
2.4.2.35
glutathione
slight stimulation
Tulipa hybrid cultivar
2.4.2.35
additional information
no stimulation by bovine serum albumin, not affected by anions like chloride and sulfate
Tulipa hybrid cultivar
2.4.2.35
NH4+
high activation
Tulipa hybrid cultivar
2.4.2.35
sucrose
slight stimulation
Tulipa hybrid cultivar
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.91
PCMB
-
Tulipa hybrid cultivar
2.4.1.159
p-chloromercuribenzoate
weak
Tulipa hybrid cultivar
2.4.2.35
Cu2+
high inhibition when applied in high concentrations
Tulipa hybrid cultivar
2.4.2.35
Mn2+
-
Tulipa hybrid cultivar
2.4.2.35
p-chloromercuribenzoate
-
Tulipa hybrid cultivar
2.4.2.35
Zn2+
high inhibition when applied in high concentrations
Tulipa hybrid cultivar
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.159
Mg2+
slight stimulation
Tulipa hybrid cultivar
2.4.1.159
additional information
no activation by Ca2+, NH4+, Mn2+, Cl- or SO42-
Tulipa hybrid cultivar
2.4.2.35
Ca2+
high activation
Tulipa hybrid cultivar
2.4.2.35
additional information
no effect by Mg2+
Tulipa hybrid cultivar
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.91
40000
-
gel filtration
Tulipa hybrid cultivar
2.4.1.159
40000
-
gel filtration
Tulipa hybrid cultivar
2.4.2.35
30000
-
gel filtration
Tulipa hybrid cultivar
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.4.1.159
UDPrhamnose + flavonol 3-O-glucoside
Tulipa hybrid cultivar
involved in flavonoid metabolism, pathway of flavonol 3-O-triglycoside biosynthesis
UDP + flavonol 3-O-rhamnosylglucoside
-
-
?
2.4.2.35
UDP-D-xylose + flavonol 3-O-glycoside
Tulipa hybrid cultivar
involved in flavonoid metabolism, pathway of flavonol 3-O-triglycoside biosynthesis
UDP + flavonol 3-O-D-xylosylglycoside
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.91
partial
Tulipa hybrid cultivar
2.4.1.159
partial
Tulipa hybrid cultivar
2.4.2.35
partial by a method that includes Sephadex G-200 chromatography, Sephadex G-15 chromatography, isoelectric focusing and DEAE-Sephadex chromatography
Tulipa hybrid cultivar
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.91
anther
-
Tulipa hybrid cultivar
-
2.4.1.159
anther
-
Tulipa hybrid cultivar
-
2.4.2.35
anther
-
Tulipa hybrid cultivar
-
2.4.2.35
pollen
at the stage of middle postmeiotic pollen ripening
Tulipa hybrid cultivar
-
2.4.2.35
tapetum
-
Tulipa hybrid cultivar
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.4.1.91
UDP-alpha-D-glucose + isorhamnetin
isorhamnetin is the best acceptor
488625
Tulipa hybrid cultivar
UDP + isorhamnetin 3-O-beta-D-glucoside
-
-
-
r
2.4.1.91
UDPgalactose + isorhamnetin
isorhamnetin is the best acceptor, 45% of the activity compared to reaction with UDPglucose
488625
Tulipa hybrid cultivar
UDP + isorhamnetin 3-O-glucoside
-
-
-
r
2.4.1.91
UDPglucose + kaempferol
45% of the activity with isorhamnetin
488625
Tulipa hybrid cultivar
UDP + kaempferol 3-O-glucoside
-
-
-
r
2.4.1.91
UDPglucose + kaempferol-4'-O-methylether
13% of the activity with isorhamnetin
488625
Tulipa hybrid cultivar
UDP + ?
-
-
-
r
2.4.1.91
UDPglucose + quercetin
93% of the activity with isorhamnetin
488625
Tulipa hybrid cultivar
UDP + quercetin 3-O-glucoside
-
-
-
r
2.4.1.91
UDPglucose + rutin
4% of the activity with isorhamnetin
488625
Tulipa hybrid cultivar
UDP + rutin 3-O-glucoside
-
-
-
r
2.4.1.159
UDPrhamnose + flavonol 3-O-diglycoside
-
488625
Tulipa hybrid cultivar
UDP + flavonol 3-O-triglycoside
-
488625
Tulipa hybrid cultivar
?
2.4.1.159
UDPrhamnose + flavonol 3-O-glucoside
acceptor substrates are 3-O-glucosides of quercetin, isorhamnetin or kaempferol, rutin, less efficient acceptors: quercetin 3-O-galactoside or quercetin 3-O-rhamnoside, kaempferol 3-O-rhamnoside or myrecetin 3-O-rhamnoside, no substrates: aglycones
488625
Tulipa hybrid cultivar
UDP + flavonol 3-O-rhamnosylglucoside
i.e. 3-O-rutinosides
488625
Tulipa hybrid cultivar
?
2.4.1.159
UDPrhamnose + flavonol 3-O-glucoside
involved in flavonoid metabolism, pathway of flavonol 3-O-triglycoside biosynthesis
488625
Tulipa hybrid cultivar
UDP + flavonol 3-O-rhamnosylglucoside
-
-
-
?
2.4.2.35
additional information
aglycones are no substrates
488625
Tulipa hybrid cultivar
?
-
-
-
?
2.4.2.35
UDP-D-xylose + flavonol 3-O-diglycoside
-
488625
Tulipa hybrid cultivar
UDP + flavonol 3-O-triglycoside
-
-
-
?
2.4.2.35
UDP-D-xylose + flavonol 3-O-glycoside
-
488625
Tulipa hybrid cultivar
UDP + flavonol 3-O-D-xylosylglycoside
-
488625
Tulipa hybrid cultivar
?
2.4.2.35
UDP-D-xylose + flavonol 3-O-glycoside
involved in flavonoid metabolism, pathway of flavonol 3-O-triglycoside biosynthesis
488625
Tulipa hybrid cultivar
UDP + flavonol 3-O-D-xylosylglycoside
-
-
-
?
2.4.2.35
UDP-D-xylose + isorhamnetin 3,7-O-diglucoside
poor substrate
488625
Tulipa hybrid cultivar
?
-
-
-
?
2.4.2.35
UDP-D-xylose + isorhamnetin 3-O-glucoside
-
488625
Tulipa hybrid cultivar
UDP + isorhamnetin 3-O-xylosylglucoside
-
-
-
?
2.4.2.35
UDP-D-xylose + kaempferol 3-O-glucoside
-
488625
Tulipa hybrid cultivar
UDP + kaempferol 3-O-xylosylglucoside
-
-
-
?
2.4.2.35
UDP-D-xylose + kaempferol 3-O-rhamnoside
poor substrate
488625
Tulipa hybrid cultivar
UDP + kaempferol 3-O-xylosylrhamnoside
-
-
-
?
2.4.2.35
UDP-D-xylose + myricetin 3-O-rhamnoside
poor substrate
488625
Tulipa hybrid cultivar
UDP + myricetin 3-O-xylosylrhamnoside
-
-
-
?
2.4.2.35
UDP-D-xylose + quercetin 3-O-arabinoglucoside
poor substrate
488625
Tulipa hybrid cultivar
UDP + quercetin 3-O-xylosylarabinoglucoside
-
-
-
?
2.4.2.35
UDP-D-xylose + quercetin 3-O-galactoside
best substrate tested
488625
Tulipa hybrid cultivar
UDP + quercetin 3-O-xylosylgalactoside
-
-
-
?
2.4.2.35
UDP-D-xylose + quercetin 3-O-glucoside
-
488625
Tulipa hybrid cultivar
UDP + quercetin 3-O-xylosylglucoside
-
-
-
?
2.4.2.35
UDP-D-xylose + quercetin 3-O-glucoside
poor substrate
488625
Tulipa hybrid cultivar
UDP + quercetin 3-O-D-xylosylglucoside
-
-
-
?
2.4.2.35
UDP-D-xylose + quercetin 3-O-rhamnoside
poor substrate
488625
Tulipa hybrid cultivar
UDP + quercetin 3-O-xylosylrhamnoside
-
488625
Tulipa hybrid cultivar
?
2.4.2.35
UDP-D-xylose + quercetin 3-O-rhamnosylglucoside
i.e. rutin, poor substrate
488625
Tulipa hybrid cultivar
UDP + quercetin 3-O-D-xylosylrhamnosylglucoside
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.91
8.5
9
-
Tulipa hybrid cultivar
2.4.1.159
8.5
9
-
Tulipa hybrid cultivar
2.4.2.35
8.5
9
-
Tulipa hybrid cultivar