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Literature summary extracted from

  • Kleinehollenhorst, G.; Behrens, H.; Pegels, G.; Strunk, N.; Wiermann, R.
    Formation of flavonol 3-O-diglycosides and flavonol 3-O-triglycosides by enzyme extracts from anthers of Tulipa cv apeldoorn - characterization and activity of 3 different O-glycosyltransferases during anther development (1982), Z. Naturforsch. C, 37c, 587-599.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.91 2-mercaptoethanol 1 mM, activation to 120% Tulipa hybrid cultivar
2.4.1.91 dithioerythritol 1 mM, activation to 124% Tulipa hybrid cultivar
2.4.1.91 glutathione 1 mM, activation to 118% Tulipa hybrid cultivar
2.4.1.91 sucrose 5%, activation to 123% Tulipa hybrid cultivar
2.4.1.159 2-mercaptoethanol slight stimulation Tulipa hybrid cultivar
2.4.1.159 dithioerythritol slight stimulation Tulipa hybrid cultivar
2.4.1.159 glutathione slight stimulation Tulipa hybrid cultivar
2.4.1.159 additional information no stimulation by sucrose or bovine serum albumin Tulipa hybrid cultivar
2.4.2.35 2-mercaptoethanol slight stimulation Tulipa hybrid cultivar
2.4.2.35 dithioerythritol slight stimulation Tulipa hybrid cultivar
2.4.2.35 glutathione slight stimulation Tulipa hybrid cultivar
2.4.2.35 additional information no stimulation by bovine serum albumin, not affected by anions like chloride and sulfate Tulipa hybrid cultivar
2.4.2.35 NH4+ high activation Tulipa hybrid cultivar
2.4.2.35 sucrose slight stimulation Tulipa hybrid cultivar

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.91 PCMB
-
 Tulipa hybrid cultivar
2.4.1.159 p-chloromercuribenzoate weak Tulipa hybrid cultivar
2.4.2.35 Cu2+ high inhibition when applied in high concentrations Tulipa hybrid cultivar
2.4.2.35 Mn2+
-
 Tulipa hybrid cultivar
2.4.2.35 p-chloromercuribenzoate
-
 Tulipa hybrid cultivar
2.4.2.35 Zn2+ high inhibition when applied in high concentrations Tulipa hybrid cultivar

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.159 Mg2+ slight stimulation Tulipa hybrid cultivar
2.4.1.159 additional information no activation by Ca2+, NH4+, Mn2+, Cl- or SO42- Tulipa hybrid cultivar
2.4.2.35 Ca2+ high activation Tulipa hybrid cultivar
2.4.2.35 additional information no effect by Mg2+ Tulipa hybrid cultivar

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.91 40000
-
 gel filtration Tulipa hybrid cultivar
2.4.1.159 40000
-
 gel filtration Tulipa hybrid cultivar
2.4.2.35 30000
-
 gel filtration Tulipa hybrid cultivar

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.159 UDPrhamnose + flavonol 3-O-glucoside Tulipa hybrid cultivar involved in flavonoid metabolism, pathway of flavonol 3-O-triglycoside biosynthesis UDP + flavonol 3-O-rhamnosylglucoside
-
 ?
2.4.2.35 UDP-D-xylose + flavonol 3-O-glycoside Tulipa hybrid cultivar involved in flavonoid metabolism, pathway of flavonol 3-O-triglycoside biosynthesis UDP + flavonol 3-O-D-xylosylglycoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.91 Tulipa hybrid cultivar
-
 cv. Apeldoorn
-
2.4.1.159 Tulipa hybrid cultivar
-
 tulip, cv. Apeldoorn
-
2.4.2.35 Tulipa hybrid cultivar
-
 tulip, cv. Apeldoorn
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.91 partial Tulipa hybrid cultivar
2.4.1.159 partial Tulipa hybrid cultivar
2.4.2.35 partial by a method that includes Sephadex G-200 chromatography, Sephadex G-15 chromatography, isoelectric focusing and DEAE-Sephadex chromatography Tulipa hybrid cultivar

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.91 anther
-
 Tulipa hybrid cultivar
-
2.4.1.159 anther
-
 Tulipa hybrid cultivar
-
2.4.2.35 anther
-
 Tulipa hybrid cultivar
-
2.4.2.35 pollen at the stage of middle postmeiotic pollen ripening Tulipa hybrid cultivar
-
2.4.2.35 tapetum
-
 Tulipa hybrid cultivar
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.91 UDP-alpha-D-glucose + isorhamnetin isorhamnetin is the best acceptor Tulipa hybrid cultivar UDP + isorhamnetin 3-O-beta-D-glucoside
-
 r
2.4.1.91 UDPgalactose + isorhamnetin isorhamnetin is the best acceptor, 45% of the activity compared to reaction with UDPglucose Tulipa hybrid cultivar UDP + isorhamnetin 3-O-glucoside
-
 r
2.4.1.91 UDPglucose + kaempferol 45% of the activity with isorhamnetin Tulipa hybrid cultivar UDP + kaempferol 3-O-glucoside
-
 r
2.4.1.91 UDPglucose + kaempferol-4'-O-methylether 13% of the activity with isorhamnetin Tulipa hybrid cultivar UDP + ?
-
 r
2.4.1.91 UDPglucose + quercetin 93% of the activity with isorhamnetin Tulipa hybrid cultivar UDP + quercetin 3-O-glucoside
-
 r
2.4.1.91 UDPglucose + rutin 4% of the activity with isorhamnetin Tulipa hybrid cultivar UDP + rutin 3-O-glucoside
-
 r
2.4.1.159 UDPrhamnose + flavonol 3-O-diglycoside
-
 Tulipa hybrid cultivar UDP + flavonol 3-O-triglycoside
-
 ?
2.4.1.159 UDPrhamnose + flavonol 3-O-glucoside acceptor substrates are 3-O-glucosides of quercetin, isorhamnetin or kaempferol, rutin, less efficient acceptors: quercetin 3-O-galactoside or quercetin 3-O-rhamnoside, kaempferol 3-O-rhamnoside or myrecetin 3-O-rhamnoside, no substrates: aglycones Tulipa hybrid cultivar UDP + flavonol 3-O-rhamnosylglucoside i.e. 3-O-rutinosides ?
2.4.1.159 UDPrhamnose + flavonol 3-O-glucoside involved in flavonoid metabolism, pathway of flavonol 3-O-triglycoside biosynthesis Tulipa hybrid cultivar UDP + flavonol 3-O-rhamnosylglucoside
-
 ?
2.4.2.35 additional information aglycones are no substrates Tulipa hybrid cultivar ?
-
 ?
2.4.2.35 UDP-D-xylose + flavonol 3-O-diglycoside
-
 Tulipa hybrid cultivar UDP + flavonol 3-O-triglycoside
-
 ?
2.4.2.35 UDP-D-xylose + flavonol 3-O-glycoside
-
 Tulipa hybrid cultivar UDP + flavonol 3-O-D-xylosylglycoside
-
 ?
2.4.2.35 UDP-D-xylose + flavonol 3-O-glycoside involved in flavonoid metabolism, pathway of flavonol 3-O-triglycoside biosynthesis Tulipa hybrid cultivar UDP + flavonol 3-O-D-xylosylglycoside
-
 ?
2.4.2.35 UDP-D-xylose + isorhamnetin 3,7-O-diglucoside poor substrate Tulipa hybrid cultivar ?
-
 ?
2.4.2.35 UDP-D-xylose + isorhamnetin 3-O-glucoside
-
 Tulipa hybrid cultivar UDP + isorhamnetin 3-O-xylosylglucoside
-
 ?
2.4.2.35 UDP-D-xylose + kaempferol 3-O-glucoside
-
 Tulipa hybrid cultivar UDP + kaempferol 3-O-xylosylglucoside
-
 ?
2.4.2.35 UDP-D-xylose + kaempferol 3-O-rhamnoside poor substrate Tulipa hybrid cultivar UDP + kaempferol 3-O-xylosylrhamnoside
-
 ?
2.4.2.35 UDP-D-xylose + myricetin 3-O-rhamnoside poor substrate Tulipa hybrid cultivar UDP + myricetin 3-O-xylosylrhamnoside
-
 ?
2.4.2.35 UDP-D-xylose + quercetin 3-O-arabinoglucoside poor substrate Tulipa hybrid cultivar UDP + quercetin 3-O-xylosylarabinoglucoside
-
 ?
2.4.2.35 UDP-D-xylose + quercetin 3-O-galactoside best substrate tested Tulipa hybrid cultivar UDP + quercetin 3-O-xylosylgalactoside
-
 ?
2.4.2.35 UDP-D-xylose + quercetin 3-O-glucoside
-
 Tulipa hybrid cultivar UDP + quercetin 3-O-xylosylglucoside
-
 ?
2.4.2.35 UDP-D-xylose + quercetin 3-O-glucoside poor substrate Tulipa hybrid cultivar UDP + quercetin 3-O-D-xylosylglucoside
-
 ?
2.4.2.35 UDP-D-xylose + quercetin 3-O-rhamnoside poor substrate Tulipa hybrid cultivar UDP + quercetin 3-O-xylosylrhamnoside
-
 ?
2.4.2.35 UDP-D-xylose + quercetin 3-O-rhamnosylglucoside i.e. rutin, poor substrate Tulipa hybrid cultivar UDP + quercetin 3-O-D-xylosylrhamnosylglucoside
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.91 8.5 9
-
 Tulipa hybrid cultivar
2.4.1.159 8.5 9
-
 Tulipa hybrid cultivar
2.4.2.35 8.5 9
-
 Tulipa hybrid cultivar