BRENDA - Enzyme Database

Dolichyl phosphate-mannosyltransferase and dolichyl phosphate-N-acetylglucosaminyltransferase activities in liver preparations from normal controls and patients with cystic fibrosis and diabetes mellitus

Alhadeff, J.A.; Watkins P.; Clin. Chim. Acta 134, 1-9 (1983)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.153
Nonidet P-40
detergent required
Homo sapiens
2.4.1.153
Triton X-100
detergent required
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.153
Mn2+
divalent cation required, 5 mM Mn2+ is optimal
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.153
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
Homo sapiens
one of the initial steps of the lipid-mediated glycosylation pathway
UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.153
Homo sapiens
-
normal controls and patients with cystic fibrosis and diabetes mellitus
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.153
liver
normal controls and patients with cystic fibrosis and diabetes mellitus
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.4.1.153
additional information
-
specific activities of dolichyl phosphate-mannosyltransferase are comparable in liver homogenates from normal controls and patients with cystic fibrosis and diabetes mellitus
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.153
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
-
488621
Homo sapiens
UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
-
-
-
?
2.4.1.153
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
one of the initial steps of the lipid-mediated glycosylation pathway
488621
Homo sapiens
UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.153
7.8
-
-
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.153
Nonidet P-40
detergent required
Homo sapiens
2.4.1.153
Triton X-100
detergent required
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.153
Mn2+
divalent cation required, 5 mM Mn2+ is optimal
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.153
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
Homo sapiens
one of the initial steps of the lipid-mediated glycosylation pathway
UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.153
liver
normal controls and patients with cystic fibrosis and diabetes mellitus
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.4.1.153
additional information
-
specific activities of dolichyl phosphate-mannosyltransferase are comparable in liver homogenates from normal controls and patients with cystic fibrosis and diabetes mellitus
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.153
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
-
488621
Homo sapiens
UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
-
-
-
?
2.4.1.153
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
one of the initial steps of the lipid-mediated glycosylation pathway
488621
Homo sapiens
UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.153
7.8
-
-
Homo sapiens