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Literature summary extracted from

  • Killick, K.A.
    Trehalose 6-phosphate synthase from Dictyostelium discoideum: partial purification and characterization of the enzyme from young sorocarps (1979), Arch. Biochem. Biophys., 196, 121-133.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.15 chondroitin sulfate activation Dictyostelium discoideum
2.4.1.15 EDTA activation Dictyostelium discoideum
2.4.1.15 heparin activation Dictyostelium discoideum
2.4.1.15 Tween 80 slight activation Dictyostelium discoideum

General Stability

EC Number General Stability Organism
2.4.1.15 stable to at least 4 successive freeze/thaw cycles Dictyostelium discoideum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.15 Cemusol NPT-12 weak inhibition Dictyostelium discoideum
2.4.1.15 Triton X-100 weak inhibition Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.15 additional information
-
 additional information complex bimodal kinetics Dictyostelium discoideum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.15 K+ maximal activation at 400 mM KCl Dictyostelium discoideum
2.4.1.15 Mg2+ activation Dictyostelium discoideum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.15 Dictyostelium discoideum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.15 partial Dictyostelium discoideum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate complex bimodal kinetics Dictyostelium discoideum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.15 sporocarp
-
 Dictyostelium discoideum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.15 6.179
-
-
 Dictyostelium discoideum

Storage Stability

EC Number Storage Stability Organism
2.4.1.15 -12°C, stable for at least 11 weeks Dictyostelium discoideum
2.4.1.15 4-6°C, 2 d, enzyme retains 75% of its activity after 2 d and 25% of its activity after 3 d Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.15 ADP-alpha-D-glucose + D-glucose 6-phosphate 13.4% of the activity with GDPglucose Dictyostelium discoideum ADP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.15 GDP-glucose + D-glucose 6-phosphate 18.4% of the activity with UDP-glucose Dictyostelium discoideum trehalose 6-phosphate + GDP
-
 ?
2.4.1.15 TDP-glucose + glucose 6-phosphate 15.9% of the activity with UDPglucose Dictyostelium discoideum trehalose 6-phosphate + TDP
-
 ?
2.4.1.15 UDPglucose + glucose 6-phosphate absolute specificity for glucose 6-phosphate Dictyostelium discoideum trehalose 6-phosphate + UDP
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.15 48
-
 5 min, 44% of its original activity is recovered Dictyostelium discoideum
2.4.1.15 55
-
 5 min, 5% of its original activity is recovered Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.15 6.5
-
-
 Dictyostelium discoideum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.15 5.5 8 pH 5.5: about 75% of maximal activity, pH 8.0: about 70% of maximal activity Dictyostelium discoideum