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Literature summary extracted from

  • Tsumori, H.; Shimamura, A.; Mukasa, H.
    Purification and properties of extracellular glucosyltransferase synthesizing 1,6-, 1,3-alpha-D-glucan from Streptococcus mutans serotype a (1985), J. Gen. Microbiol., 131, 3347-3353.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.125 Dextran T10 no stimulation in the range 0.01-2.0 mg/ml Streptococcus mutans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.125 4.9
-
 sucrose pH 6.5, 37°C Streptococcus mutans
2.4.1.140 4.9
-
 sucrose
-
 Streptococcus mutans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.125 extracellular
-
 Streptococcus mutans
-
-
2.4.1.140 extracellular
-
 Streptococcus mutans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.125 159000
-
 1 * 159000, SDS-PAGE Streptococcus mutans
2.4.1.140 159000
-
 x * 159000, SDS-PAGE Streptococcus mutans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.125 Streptococcus mutans
-
 HS6, serotype a
-
2.4.1.140 Streptococcus mutans
-
 HS6 serotype a
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.125 additional information 1.5% Streptococcus mutans
2.4.1.140 glycoprotein 1.5% carbohydrate Streptococcus mutans

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.125 HS6, serotype a Streptococcus mutans
2.4.1.140
-
 Streptococcus mutans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.125 culture supernatant
-
 Streptococcus mutans
-
2.4.1.140 culture medium
-
 Streptococcus mutans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.125 89.7
-
-
 Streptococcus mutans
2.4.1.140 89.7
-
-
 Streptococcus mutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.125 sucrose + (1,6-alpha-D-glucosyl)n also transfers glucosyl residues to the 3-position on glucose residues in glucans, producing a highly-branched 1,6-alpha-D-glucan Streptococcus mutans D-fructose + (1,6-alpha-D-glucosyl)n+1 glucan consists of 49.1 mol% 1,6-alpha-linked glucose and 33.9 mol% 1,3-alpha-linked glucose with 13.6 mol% terminal glucose and 3.3 mol% 1,3,6-alpha-branched glucose ?
2.4.1.140 sucrose + alpha-D-glucan
-
 Streptococcus mutans alternating-1,6-1,3-alpha-D-glucan glucan consists of 49.1 mol% 1,6-alpha-linked glucose and 33.9 mol% 1,3-alpha-linked glucose with 13.6 mol% terminal glucose and 3.3 mol% 1,3,6-alpha-branched glucose ?

Subunits

EC Number Subunits Comment Organism
2.4.1.125 monomer 1 * 159000, SDS-PAGE Streptococcus mutans
2.4.1.140 ? x * 159000, SDS-PAGE Streptococcus mutans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.125 37
-
 assay at Streptococcus mutans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.125 6
-
-
 Streptococcus mutans
2.4.1.140 6
-
-
 Streptococcus mutans