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Literature summary extracted from

  • Douglas, R.H.; Ballou, C.E.
    Purification of an alpha-N-acetylglucosaminyltransferase from the yeast Kluyveromyces lactis and a study of mutants defective in this enzyme activity (1982), Biochemistry, 21, 1561-1570.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.138 additional information analysis of mutations in strain mnn2-2 Kluyveromyces lactis

General Stability

EC Number General Stability Organism
2.4.1.138 Triton X-100 stabilizes solubilized enzyme Kluyveromyces lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.138 Co2+ strong, 1 mM, even in the presence of Mn2+, wild-type Kluyveromyces lactis
2.4.1.138 Cu2+ wild-type Kluyveromyces lactis
2.4.1.138 imidazole wild-type Kluyveromyces lactis
2.4.1.138 Mn2+ above 10 mM, wild-type Kluyveromyces lactis
2.4.1.138 NaCl weak Kluyveromyces lactis
2.4.1.138 NaN3
-
 Kluyveromyces lactis
2.4.1.138 phosphate wild-type, complete inhibition at 40 mM Kluyveromyces lactis
2.4.1.138 Tris wild-type Kluyveromyces lactis
2.4.1.138 Tween 80
-
 Kluyveromyces lactis
2.4.1.138 UDP wild-type Kluyveromyces lactis
2.4.1.138 UDP-hexylamine wild-type Kluyveromyces lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.138 0.0364
-
 UDP-N-acetylglucosamine mutant 2-2 Kluyveromyces lactis
2.4.1.138 0.0447
-
 UDP-N-acetylglucosamine wild-type Kluyveromyces lactis
2.4.1.138 11
-
 1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose solubilized enzyme Kluyveromyces lactis
2.4.1.138 13
-
 1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose membrane-bound enzyme Kluyveromyces lactis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.138 endoplasmic reticulum integral membrane protein Kluyveromyces lactis 5783
-
2.4.1.138 protoplast
-
 Kluyveromyces lactis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.138 Mn2+ requirement, 10 mM, inhibits at higher concentrations, Mg2+ or Ca2+ cannot replace Mn2+ Kluyveromyces lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.138 300000
-
 gel filtration in the presence of Triton X-100, native MW may be lower Kluyveromyces lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.138 UDP-N-acetyl-D-glucosamine + 1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose Kluyveromyces lactis involved in biosynthesis of mannoprotein side-chain units UDP + 1,3-alpha-D-mannosyl-1,2-(N-acetyl-alpha-D-glucosaminyl-alpha-D-mannosyl)-1,2-alpha-D-mannosyl-D-mannose
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.4.1.138 additional information organic solvent extraction, unstable to Kluyveromyces lactis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.138 Kluyveromyces lactis
-
 yeast, wild-type strains Y-58a his 4c or his 3 and mutant strains mnn1, mnn2-1 or mnn2-2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.138 glycoprotein possesses no N-acetylglucosamine residues, enzyme does not bind to wheat germ agglutinin Kluyveromyces lactis

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.138 partial Kluyveromyces lactis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.138 additional information
-
 activity of wild-type and mutant strains with different acceptors Kluyveromyces lactis
2.4.1.138 0.0056
-
-
 Kluyveromyces lactis

Storage Stability

EC Number Storage Stability Organism
2.4.1.138 4°C, in imidazole-glycerol buffer, 2% Triton X-100, t1/2: 80 days Kluyveromyces lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.138 UDP-N-acetyl-alpha-D-glucosamine + 1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose
-
 Kluyveromyces lactis UDP + 1,3-alpha-D-mannosyl-1,2-(N-acetyl-alpha-D-glucosaminyl-alpha-D-mannosyl)-1,2-alpha-D-mannosyl-D-mannose
-
 ?
2.4.1.138 UDP-N-acetyl-D-glucosamine + 1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose i.e. mannotetraose acceptor: acceptor substrates are mannoprotein, saccharides with more than 25 mannose-units, with 15 to 25 units, with 8 to 15 units, with 5 to 8 units and below 5 units, the latter being the best substrates Kluyveromyces lactis UDP + 1,3-alpha-D-mannosyl-1,2-(N-acetyl-alpha-D-glucosaminyl-alpha-D-mannosyl)-1,2-alpha-D-mannosyl-D-mannose
-
 ?
2.4.1.138 UDP-N-acetyl-D-glucosamine + 1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose involved in biosynthesis of mannoprotein side-chain units Kluyveromyces lactis UDP + 1,3-alpha-D-mannosyl-1,2-(N-acetyl-alpha-D-glucosaminyl-alpha-D-mannosyl)-1,2-alpha-D-mannosyl-D-mannose
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.138 35
-
 2 h, inactivation Kluyveromyces lactis
2.4.1.138 45
-
 t1/2: 4 min Kluyveromyces lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.138 additional information
-
 pI: 4.9, in the presence of Triton X-100 and 2 M urea Kluyveromyces lactis
2.4.1.138 6.5
-
-
 Kluyveromyces lactis