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Literature summary extracted from

  • Gross, K.C.; Pharr, D.M.
    Cucumber fruit sucrose synthetase isozymes (1982), Phytochemistry, 21, 1241-1244.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.13 UDP strong Cucumis sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.13 0.3 0.38 UDPglucose isozyme SS2 Cucumis sativus
2.4.1.13 5.3 6 UDPglucose cosubstrate UDPfructose, isozyme SS2 Cucumis sativus
2.4.1.13 11.5
-
 D-fructose isozyme SS1 Cucumis sativus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.13 Mn2+ activation Cucumis sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.13 540000
-
 gel filtration Cucumis sativus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.13 Cucumis sativus
-
 cucumber, isoforms SSI, SSII
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.13 2 isozymes: SS1 and SS2, partial Cucumis sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.13 fruit
-
 Cucumis sativus
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.13 4°C, isozyme SS1, 4 months, isozyme SS2, t1/2: 3 weeks Cucumis sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.13 ADP-glucose + D-fructose
-
 Cucumis sativus ADP + sucrose
-
 r
2.4.1.13 UDP + sucrose
-
 Cucumis sativus UDP-glucose + D-fructose
-
 ?
2.4.1.13 UDP-alpha-D-glucose + D-fructose
-
 Cucumis sativus UDP + sucrose
-
 ?
2.4.1.13 UDPglucose + D-fructose
-
 Cucumis sativus UDP + sucrose
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.13 6
-
 sucrose cleavage Cucumis sativus
2.4.1.13 7.5
-
 sucrose synthesis, isozyme SS2 Cucumis sativus
2.4.1.13 9
-
 sucrose synthesis Cucumis sativus
2.4.1.13 9
-
 isozyme SS Cucumis sativus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.13 5 10 about half-maximal activity at pH 5.0 and 10.0, sucrose synthesis Cucumis sativus
2.4.1.13 5 8 about half-maximal activity at pH 5.0 and 8.0, sucrose cleavage Cucumis sativus