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Literature summary extracted from

  • Morell, M.; Copeland, L.
    Sucrose synthase of soybean nodules (1985), Plant Physiol., 78, 149-154.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.13 allantoin activation, sucrose synthesis Glycine max

General Stability

EC Number General Stability Organism
2.4.1.13 2-mercaptoethanol stabilizes Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.13 AMP not inhibitory Glycine max
2.4.1.13 ATP sucrose synthesis Glycine max
2.4.1.13 fructose
-
 Glycine max
2.4.1.13 glucose
-
 Glycine max
2.4.1.13 imidazole
-
 Glycine max
2.4.1.13 additional information not inhibitory: DTT, GSH, 2-mercaptoethanol, EDTA; not inhibitory: fructose 6-phosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 1,6-diphosphate; not inhibitory: galactose, mannose, maltose, raffinose, 3-phosphoglycerate, phosphoenolpyruvate, ethanol, succinate, 2-oxoglutarate, glutamine, NAD+, diphosphate Glycine max
2.4.1.13 Tris sucrose cleavage Glycine max
2.4.1.13 UDP strong Glycine max
2.4.1.13 UDPglucose
-
 Glycine max
2.4.1.13 UTP synthesis Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.13 0.005
-
 UDP
-
 Glycine max
2.4.1.13 0.012 0.033 UDPglucose
-
 Glycine max
2.4.1.13 0.13
-
 ADP
-
 Glycine max
2.4.1.13 0.98 1.66 fructose cosubstrate CDP Glycine max
2.4.1.13 0.98 1.66 fructose cosubstrate ADPglucose Glycine max
2.4.1.13 3.7 5 fructose
-
 Glycine max
2.4.1.13 16.9 40 sucrose
-
 Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.13 cytosol
-
 Glycine max 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.13 Ca2+ activation of sucrose synthesis Glycine max
2.4.1.13 Ca2+ slight activation of sucrose cleavage at 10 mM Glycine max
2.4.1.13 Mg2+ activation Glycine max
2.4.1.13 Mg2+ slight activation of sucrose cleavage Glycine max
2.4.1.13 Mn2+ activation Glycine max
2.4.1.13 additional information no activation by K+, Na+, NH4+, Cl-, Br-, F-, NO3-, phosphate, sulfate, borate, acetate, citrate Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.13 90000
-
 4 * 90000, SDS-PAGE Glycine max
2.4.1.13 380000
-
 SDS-PAGE Glycine max
2.4.1.13 412000
-
 gel filtration Glycine max

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.13 Glycine max
-
 Merr cv. Williams
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.13
-
 Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.13 nodule nodulaid inoculum: Rhizobium japonicum CB 1809 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.13 15.1
-
-
 Glycine max

Storage Stability

EC Number Storage Stability Organism
2.4.1.13 4°C, 20 mM potassium phosphate buffer, pH 7.0, 5 mM 2-mercaptoethanol, 4 weeks stable Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.13 ADP-glucose + D-fructose
-
 Glycine max ADP + sucrose
-
 r
2.4.1.13 CDP-glucose + D-fructose
-
 Glycine max CDP + sucrose
-
 r
2.4.1.13 GDP-glucose + D-fructose
-
 Glycine max GDP + sucrose
-
 r
2.4.1.13 UDP + sucrose also substrate: ADP Glycine max UDP-glucose + D-fructose
-
 ?
2.4.1.13 UDP + sucrose substrate: CDP Glycine max UDP-glucose + D-fructose
-
 ?
2.4.1.13 UDP + sucrose ADP is less effective Glycine max UDP-glucose + D-fructose
-
 ?
2.4.1.13 UDPglucose + D-fructose
-
 Glycine max UDP + sucrose
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.13 tetramer 4 * 90000, SDS-PAGE Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.13 6
-
 sucrose cleavage Glycine max
2.4.1.13 9.5
-
 sucrose synthesis Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.13 5 8.2 about 90% of maximal activity at pH 5.0 and about half-maximal activity at pH 8.2, sucrose cleavage Glycine max
2.4.1.13 7.5 10 about half-maximal activity at pH 7.5 and about 90% of maximal activity at pH 10.0, sucrose synthesis Glycine max