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Literature summary extracted from

  • Nurmann, G.; Strack, D.
    Formation of 1-sinapoylglucose by UDP-glucose: sinapic acid glucosyltransferase from cotyledons of Raphanus sativus (1981), Z. Pflanzenphysiol., 102, 11-17.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.120 additional information SH-group required Raphanus sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.120 0.03
-
 Sinapic acid
-
 Raphanus sativus
2.4.1.120 0.055
-
 UDPglucose
-
 Raphanus sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.120 UDP-alpha-D-glucose + sinapic acid Raphanus sativus
-
 UDP + 1-O-sinapoyl-beta-D-glucose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.120 Raphanus sativus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.120 partial Raphanus sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.120 seedling
-
 Raphanus sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.120 0.0094
-
 reaction with sinapic acid Raphanus sativus

Storage Stability

EC Number Storage Stability Organism
2.4.1.120 4°C, 8 days, 23% loss of activity Raphanus sativus
2.4.1.120 enzyme is less stable at -20°C than at 4°C Raphanus sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.120 dTDP-alpha-D-glucose + sinapic acid
-
 Raphanus sativus dTDP + 1-O-sinapoyl-beta-D-glucose
-
 ?
2.4.1.120 UDP-alpha-D-glucose + sinapic acid
-
 Raphanus sativus UDP + 1-O-sinapoyl-beta-D-glucose
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.120 5.8
-
 potassium phosphate buffer Raphanus sativus
2.4.1.120 6
-
 MES buffer Raphanus sativus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.120 5 7 pH 5.0: 85% of maximal activity, pH 7.0: 63% of maximal activity Raphanus sativus