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Literature summary extracted from

  • Knauer, R.; Lehle, L.
    The oligosaccharyltransferase complex from yeast (1999), Biochim. Biophys. Acta, 1426, 259-273.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.99.18 strongly stabilized by addition of phospholipids upon detergent solubilization, phosphatidylcholine is twice as effective as phosphatidylethanolamine Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.99.18 membrane
-
 Sus scrofa 16020
-
2.4.99.18 membrane bound to Saccharomyces cerevisiae 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.99.18 Mg2+
-
 Gallus gallus
2.4.99.18 Mg2+ absolute requirement for divalent cations that can adopt an octahedral coordination geometry with preference for Mn2+ over Mg2+ Saccharomyces cerevisiae
2.4.99.18 Mn2+
-
 Gallus gallus
2.4.99.18 Mn2+ absolute requirement for divalent cations that can adopt an octahedral coordination geometry with preference for Mn2+ over Mg2+ Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.99.18 6000
-
 x * 62000-64000, Ost1p, + x * 6000, Stt3p, + x * 47000, Wbp1p, + x * 34000, Ost3p, + x * 32000, Ost6p, + x * 30000, Swp1p, + x * 16000, Ost2p, + x * 9500, Ost5p, + x * 3600, Ost4p Saccharomyces cerevisiae
2.4.99.18 9500
-
 x * 62000-64000, Ost1p, + x * 6000, Stt3p, + x * 47000, Wbp1p, + x * 34000, Ost3p, + x * 32000, Ost6p, + x * 30000, Swp1p, + x * 16000, Ost2p, + x * 9500, Ost5p, + x * 3600, Ost4p Saccharomyces cerevisiae
2.4.99.18 16000
-
 x * 62000-64000, Ost1p, + x * 6000, Stt3p, + x * 47000, Wbp1p, + x * 34000, Ost3p, + x * 32000, Ost6p, + x * 30000, Swp1p, + x * 16000, Ost2p, + x * 9500, Ost5p, + x * 3600, Ost4p Saccharomyces cerevisiae
2.4.99.18 30000
-
 x * 62000-64000, Ost1p, + x * 6000, Stt3p, + x * 47000, Wbp1p, + x * 34000, Ost3p, + x * 32000, Ost6p, + x * 30000, Swp1p, + x * 16000, Ost2p, + x * 9500, Ost5p, + x * 3600, Ost4p Saccharomyces cerevisiae
2.4.99.18 32000
-
 x * 62000-64000, Ost1p, + x * 6000, Stt3p, + x * 47000, Wbp1p, + x * 34000, Ost3p, + x * 32000, Ost6p, + x * 30000, Swp1p, + x * 16000, Ost2p, + x * 9500, Ost5p, + x * 3600, Ost4p Saccharomyces cerevisiae
2.4.99.18 34000
-
 x * 62000-64000, Ost1p, + x * 6000, Stt3p, + x * 47000, Wbp1p, + x * 34000, Ost3p, + x * 32000, Ost6p, + x * 30000, Swp1p, + x * 16000, Ost2p, + x * 9500, Ost5p, + x * 3600, Ost4p Saccharomyces cerevisiae
2.4.99.18 47000
-
 x * 62000-64000, Ost1p, + x * 6000, Stt3p, + x * 47000, Wbp1p, + x * 34000, Ost3p, + x * 32000, Ost6p, + x * 30000, Swp1p, + x * 16000, Ost2p, + x * 9500, Ost5p, + x * 3600, Ost4p Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.99.18 lipid-linked oligosaccharide + unfolded nascent polypeptide chain Gallus gallus
-
 ?
-
 ?
2.4.99.18 lipid-linked oligosaccharide + unfolded nascent polypeptide chain Canis lupus familiaris
-
 ?
-
 ?
2.4.99.18 lipid-linked oligosaccharide + unfolded nascent polypeptide chain Saccharomyces cerevisiae N-glycosylation of eukaryotic secretory and membrane-bound proteins is an essential and highly conserved protein modification. The key step in this pathway is the en bloc transfer of the high mannose core oligosaccharide Gly3Man9GlcNAc2 from the lipid carries dolichyl phosphate to selected Asn-X-Ser/Thr sequences of nascent polypeptide chains during their translocation across the endoplasmic reticulum membrane ?
-
 ?
2.4.99.18 lipid-linked oligosaccharide + unfolded nascent polypeptide chain Saccharomyces cerevisiae dolichyl-diphosphochitobiose-Man9Glc3 is the preferred glycosyl donor both in vivo and in vitro ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.99.18 Canis lupus familiaris
-
-
-
2.4.99.18 Gallus gallus
-
-
-
2.4.99.18 Homo sapiens
-
-
-
2.4.99.18 Saccharomyces cerevisiae P48439
-
-
2.4.99.18 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.99.18 liver
-
 Gallus gallus
-
2.4.99.18 liver
-
 Sus scrofa
-
2.4.99.18 oviduct
-
 Gallus gallus
-
2.4.99.18 pancreas
-
 Canis lupus familiaris
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.99.18 dolichyl diphosphooligosaccharide + protein L-asparagine
-
 Gallus gallus dolichyl diphosphate + glycoprotein with the oligosaccharide chain attached by N-glycosyl-linkage to protein L-asparagine
-
 ?
2.4.99.18 dolichyl diphosphooligosaccharide + protein L-asparagine
-
 Homo sapiens dolichyl diphosphate + glycoprotein with the oligosaccharide chain attached by N-glycosyl-linkage to protein L-asparagine
-
 ?
2.4.99.18 dolichyl diphosphooligosaccharide + protein L-asparagine
-
 Sus scrofa dolichyl diphosphate + glycoprotein with the oligosaccharide chain attached by N-glycosyl-linkage to protein L-asparagine
-
 ?
2.4.99.18 dolichyl diphosphooligosaccharide + protein L-asparagine
-
 Canis lupus familiaris dolichyl diphosphate + glycoprotein with the oligosaccharide chain attached by N-glycosyl-linkage to protein L-asparagine
-
 ?
2.4.99.18 dolichyl diphosphooligosaccharide + protein L-asparagine dolichyl-diphosphochitobiose-Man9Glc3 is the preferred glycosyl donor both in vivo and in vitro. The minimal glycosyl donor is dolichyl-diphosphochitobiose Saccharomyces cerevisiae dolichyl diphosphate + glycoprotein with the oligosaccharide chain attached by N-glycosyl-linkage to protein L-asparagine
-
 ?
2.4.99.18 lipid-linked oligosaccharide + unfolded nascent polypeptide chain
-
 Gallus gallus ?
-
 ?
2.4.99.18 lipid-linked oligosaccharide + unfolded nascent polypeptide chain
-
 Canis lupus familiaris ?
-
 ?
2.4.99.18 lipid-linked oligosaccharide + unfolded nascent polypeptide chain N-glycosylation of eukaryotic secretory and membrane-bound proteins is an essential and highly conserved protein modification. The key step in this pathway is the en bloc transfer of the high mannose core oligosaccharide Gly3Man9GlcNAc2 from the lipid carries dolichyl phosphate to selected Asn-X-Ser/Thr sequences of nascent polypeptide chains during their translocation across the endoplasmic reticulum membrane Saccharomyces cerevisiae ?
-
 ?
2.4.99.18 lipid-linked oligosaccharide + unfolded nascent polypeptide chain dolichyl-diphosphochitobiose-Man9Glc3 is the preferred glycosyl donor both in vivo and in vitro Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.99.18 ?
-
 Gallus gallus
2.4.99.18 ?
-
 Sus scrofa
2.4.99.18 ?
-
 Canis lupus familiaris
2.4.99.18 ? x * 62000-64000, Ost1p, + x * 6000, Stt3p, + x * 47000, Wbp1p, + x * 34000, Ost3p, + x * 32000, Ost6p, + x * 30000, Swp1p, + x * 16000, Ost2p, + x * 9500, Ost5p, + x * 3600, Ost4p Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.99.18 6.5 7.5
-
 Saccharomyces cerevisiae