BRENDA - Enzyme Database

Biosynthesis of glycoproteins in Candida albicans: Activity of mannosyl and glucosyl transferases

Arroyo-Flores, B.L.; Rodriguez-Bonilla, J.; Villagomez-Castro, J.C.; Calvo-Mendez, C.; Flores-Carreon, A.; Lopez-Romero, E.; Fungal Genet. Biol. 30, 127-133 (2000)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.117
Amphomycin
IC50 0.04 mM
Candida albicans
2.4.1.117
Ca2+
slight inhibition
Candida albicans
2.4.1.117
Mn2+
slight inhibition
Candida albicans
2.4.1.117
UMP
-
Candida albicans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.117
0.05
-
UDP-glucose
-
Candida albicans
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.117
Ca2+
no stimulation
Candida albicans
2.4.1.117
Mg2+
Mg2+ required, not Mn2+, Ca2+
Candida albicans
2.4.1.117
Mn2+
no stimulation
Candida albicans
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.117
Candida albicans
-
strain ATCC 26555
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.117
UDP-glucose + dolichyl phosphate
-
488488
Candida albicans
UDP + dolichyl beta-D-glucosyl phosphate
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.117
28
-
-
Candida albicans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.117
7.5
-
Tris/HCl buffer
Candida albicans
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.4.1.117
0.3
-
UMP
-
Candida albicans
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.4.1.117
0.04
-
IC50 0.04 mM
Candida albicans
Amphomycin
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.4.1.117
0.04
-
IC50 0.04 mM
Candida albicans
Amphomycin
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.117
Amphomycin
IC50 0.04 mM
Candida albicans
2.4.1.117
Ca2+
slight inhibition
Candida albicans
2.4.1.117
Mn2+
slight inhibition
Candida albicans
2.4.1.117
UMP
-
Candida albicans
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.4.1.117
0.3
-
UMP
-
Candida albicans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.117
0.05
-
UDP-glucose
-
Candida albicans
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.117
Ca2+
no stimulation
Candida albicans
2.4.1.117
Mg2+
Mg2+ required, not Mn2+, Ca2+
Candida albicans
2.4.1.117
Mn2+
no stimulation
Candida albicans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.117
UDP-glucose + dolichyl phosphate
-
488488
Candida albicans
UDP + dolichyl beta-D-glucosyl phosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.117
28
-
-
Candida albicans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.117
7.5
-
Tris/HCl buffer
Candida albicans