BRENDA - Enzyme Database

Characterization of maize endosperm UDP-glucose:dolicholphosphate glucosyltransferase

Miernyk, J.A.; Riedell, W.E.; Phytochemistry 30, 2865-2867 (1991)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.117
detergent
required for maximum activity, optimum for Triton X-100: 0.015%
Zea mays
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.117
tunicamycin
50% inhibition at 0.04 mg per ml
Zea mays
2.4.1.117
UDP
-
Zea mays
2.4.1.117
UDP-glucuronic acid
-
Zea mays
2.4.1.117
UMP
-
Zea mays
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.117
additional information
-
additional information
-
Zea mays
2.4.1.117
0.0005
-
UDP-glucose
-
Zea mays
2.4.1.117
0.0012
-
dolichyl phosphate
-
Zea mays
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.117
microsome
-
Zea mays
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.117
Mg2+
Km: 0.7 mM
Zea mays
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.117
Zea mays
-
L. inbred A 636
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.117
partial
Zea mays
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.117
endosperm
-
Zea mays
-
Storage Stability
EC Number
Storage Stability
Organism
2.4.1.117
-70°C, up to 4 weeks
Zea mays
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.117
UDP-glucose + dolichyl phosphate
-
488487
Zea mays
UDP + dolichyl beta-D-glucosyl phosphate
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.117
7.5
-
-
Zea mays
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.4.1.117
0.0076
-
UDP-glucuronic acid
-
Zea mays
2.4.1.117
0.15
-
UDP
-
Zea mays
2.4.1.117
0.65
-
UMP
-
Zea mays
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.117
detergent
required for maximum activity, optimum for Triton X-100: 0.015%
Zea mays
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.117
tunicamycin
50% inhibition at 0.04 mg per ml
Zea mays
2.4.1.117
UDP
-
Zea mays
2.4.1.117
UDP-glucuronic acid
-
Zea mays
2.4.1.117
UMP
-
Zea mays
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.4.1.117
0.0076
-
UDP-glucuronic acid
-
Zea mays
2.4.1.117
0.15
-
UDP
-
Zea mays
2.4.1.117
0.65
-
UMP
-
Zea mays
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.117
additional information
-
additional information
-
Zea mays
2.4.1.117
0.0005
-
UDP-glucose
-
Zea mays
2.4.1.117
0.0012
-
dolichyl phosphate
-
Zea mays
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.117
microsome
-
Zea mays
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.117
Mg2+
Km: 0.7 mM
Zea mays
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.117
partial
Zea mays
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.117
endosperm
-
Zea mays
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.1.117
-70°C, up to 4 weeks
Zea mays
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.117
UDP-glucose + dolichyl phosphate
-
488487
Zea mays
UDP + dolichyl beta-D-glucosyl phosphate
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.117
7.5
-
-
Zea mays