BRENDA - Enzyme Database

Partial purificaton, photoaffinity labeling, and properties of mung bean UDP-glucose:dolicholphosphate glucosyltransferase

Drake, R.R.; Kaushal, G.P.; Pastuszak, I.; Elbein, A.D.; Plant Physiol. 97, 396-401 (1991)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.117
EDTA
-
Vigna radiata var. radiata
2.4.1.117
MnCl2
stimulates at 1 mM, inhibits at higher concentration
Vigna radiata var. radiata
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.117
0.002
-
dolichyl phosphate
-
Vigna radiata var. radiata
2.4.1.117
0.027
-
UDP-glucose
-
Vigna radiata var. radiata
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.117
microsome
-
Vigna radiata var. radiata
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.117
Ca2+
CaCl2 cannot replace MgCl2 or MnCl2
Vigna radiata var. radiata
2.4.1.117
Mn2+
MnCl2 stimulates at 1 mM, inhibits at higher concentration
Vigna radiata var. radiata
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.117
39000
-
x * 39000, SDS-PAGE after photoaffinity labeling, catalytic subunit
Vigna radiata var. radiata
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.117
additional information
Vigna radiata var. radiata
enzyme is involved in the biosynthesis of the lipid-linked oligosaccharides that are precursors of N-linked glycoproteins
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.117
Vigna radiata var. radiata
-
mung bean
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.117
partial
Vigna radiata var. radiata
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.117
additional information
-
-
Vigna radiata var. radiata
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.117
additional information
enzyme is involved in the biosynthesis of the lipid-linked oligosaccharides that are precursors of N-linked glycoproteins
488486
Vigna radiata var. radiata
?
-
-
-
-
2.4.1.117
UDP-glucose + dolichyl phosphate
-
488486
Vigna radiata var. radiata
UDP + dolichyl beta-D-glucosyl phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.4.1.117
?
x * 39000, SDS-PAGE after photoaffinity labeling, catalytic subunit
Vigna radiata var. radiata
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.117
37
-
assay at
Vigna radiata var. radiata
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.117
6
7
-
Vigna radiata var. radiata
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.117
EDTA
-
Vigna radiata var. radiata
2.4.1.117
MnCl2
stimulates at 1 mM, inhibits at higher concentration
Vigna radiata var. radiata
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.117
0.002
-
dolichyl phosphate
-
Vigna radiata var. radiata
2.4.1.117
0.027
-
UDP-glucose
-
Vigna radiata var. radiata
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.117
microsome
-
Vigna radiata var. radiata
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.117
Ca2+
CaCl2 cannot replace MgCl2 or MnCl2
Vigna radiata var. radiata
2.4.1.117
Mn2+
MnCl2 stimulates at 1 mM, inhibits at higher concentration
Vigna radiata var. radiata
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.117
39000
-
x * 39000, SDS-PAGE after photoaffinity labeling, catalytic subunit
Vigna radiata var. radiata
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.117
additional information
Vigna radiata var. radiata
enzyme is involved in the biosynthesis of the lipid-linked oligosaccharides that are precursors of N-linked glycoproteins
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.117
partial
Vigna radiata var. radiata
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.117
additional information
-
-
Vigna radiata var. radiata
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.117
additional information
enzyme is involved in the biosynthesis of the lipid-linked oligosaccharides that are precursors of N-linked glycoproteins
488486
Vigna radiata var. radiata
?
-
-
-
-
2.4.1.117
UDP-glucose + dolichyl phosphate
-
488486
Vigna radiata var. radiata
UDP + dolichyl beta-D-glucosyl phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.4.1.117
?
x * 39000, SDS-PAGE after photoaffinity labeling, catalytic subunit
Vigna radiata var. radiata
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.117
37
-
assay at
Vigna radiata var. radiata
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.117
6
7
-
Vigna radiata var. radiata