BRENDA - Enzyme Database

Glycoprotein synthesis in maize endosperm cells. The nucleoside diphosphate-sugar:dolichol-phosphate glycosyltransferase

Riedell, W.E.; Miernyk, J.A.; Plant Physiol. 87, 420-426 (1988)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.117
detergent
required for maximum activity, optimum for Triton X-100: 0.015%
Zea mays
2.4.1.117
Triton X-100
required for optimal activity, 0.01-0.015%
Zea mays
General Stability
EC Number
General Stability
Organism
2.4.1.83
the enzyme is resistant to inactivation by incubation with trypsin unless Triton is present
Zea mays
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.83
GDP
-
Zea mays
2.4.1.83
GMP
-
Zea mays
2.4.1.83
tunicamycin
-
Zea mays
2.4.1.117
tunicamycin
-
Zea mays
2.4.1.117
UDP
-
Zea mays
2.4.1.117
UMP
-
Zea mays
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.00173
-
GDPmannose
-
Zea mays
2.4.1.117
additional information
-
additional information
-
Zea mays
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.83
microsome
-
Zea mays
-
-
2.4.1.117
microsome
membrane
Zea mays
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.83
Mg2+
Km: 1.31 mM
Zea mays
2.4.1.117
Ca2+
divalent cation required, efficiency of activiation in descending order: Mg2+, Mn2+, Ca2+, Co2+, Zn2+
Zea mays
2.4.1.117
Co2+
divalent cation required, efficiency of activiation in descending order: Mg2+, Mn2+, Ca2+, Co2+, Zn2+
Zea mays
2.4.1.117
Mg2+
divalent cation required, efficiency of activiation in descending order: Mg2+, Mn2+, Ca2+, Co2+, Zn2+; Km: 0.7 mM
Zea mays
2.4.1.117
Mn2+
divalent cation required, efficiency of activiation in descending order: Mg2+, Mn2+, Ca2+, Co2+, Zn2+
Zea mays
2.4.1.117
Zn2+
divalent cation required, efficiency of activiation in descending order: Mg2+, Mn2+, Ca2+, Co2+, Zn2+
Zea mays
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.83
Zea mays
-
L. inbred A636
-
2.4.1.117
Zea mays
-
L. inbred A 636
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.83
cell culture
-
Zea mays
-
2.4.1.83
endosperm
-
Zea mays
-
2.4.1.117
endosperm
culture
Zea mays
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
488483
Zea mays
GDP + dolichyl D-mannosyl phosphate
-
488483
Zea mays
?
2.4.1.117
UDP-glucose + dolichyl phosphate
-
488483
Zea mays
UDP + dolichyl beta-D-glucosyl phosphate
-
488483
Zea mays
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.83
7
-
-
Zea mays
2.4.1.117
6.5
-
-
Zea mays
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.4.1.83
0.018
-
GDP
-
Zea mays
2.4.1.83
0.235
-
GMP
-
Zea mays
2.4.1.117
0.14
-
UDP
-
Zea mays
2.4.1.117
0.66
-
UMP
-
Zea mays
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.117
detergent
required for maximum activity, optimum for Triton X-100: 0.015%
Zea mays
2.4.1.117
Triton X-100
required for optimal activity, 0.01-0.015%
Zea mays
General Stability (protein specific)
EC Number
General Stability
Organism
2.4.1.83
the enzyme is resistant to inactivation by incubation with trypsin unless Triton is present
Zea mays
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.83
GDP
-
Zea mays
2.4.1.83
GMP
-
Zea mays
2.4.1.83
tunicamycin
-
Zea mays
2.4.1.117
tunicamycin
-
Zea mays
2.4.1.117
UDP
-
Zea mays
2.4.1.117
UMP
-
Zea mays
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.4.1.83
0.018
-
GDP
-
Zea mays
2.4.1.83
0.235
-
GMP
-
Zea mays
2.4.1.117
0.14
-
UDP
-
Zea mays
2.4.1.117
0.66
-
UMP
-
Zea mays
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.00173
-
GDPmannose
-
Zea mays
2.4.1.117
additional information
-
additional information
-
Zea mays
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.83
microsome
-
Zea mays
-
-
2.4.1.117
microsome
membrane
Zea mays
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.83
Mg2+
Km: 1.31 mM
Zea mays
2.4.1.117
Ca2+
divalent cation required, efficiency of activiation in descending order: Mg2+, Mn2+, Ca2+, Co2+, Zn2+
Zea mays
2.4.1.117
Co2+
divalent cation required, efficiency of activiation in descending order: Mg2+, Mn2+, Ca2+, Co2+, Zn2+
Zea mays
2.4.1.117
Mg2+
divalent cation required, efficiency of activiation in descending order: Mg2+, Mn2+, Ca2+, Co2+, Zn2+; Km: 0.7 mM
Zea mays
2.4.1.117
Mn2+
divalent cation required, efficiency of activiation in descending order: Mg2+, Mn2+, Ca2+, Co2+, Zn2+
Zea mays
2.4.1.117
Zn2+
divalent cation required, efficiency of activiation in descending order: Mg2+, Mn2+, Ca2+, Co2+, Zn2+
Zea mays
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.83
cell culture
-
Zea mays
-
2.4.1.83
endosperm
-
Zea mays
-
2.4.1.117
endosperm
culture
Zea mays
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
488483
Zea mays
GDP + dolichyl D-mannosyl phosphate
-
488483
Zea mays
?
2.4.1.117
UDP-glucose + dolichyl phosphate
-
488483
Zea mays
UDP + dolichyl beta-D-glucosyl phosphate
-
488483
Zea mays
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.83
7
-
-
Zea mays
2.4.1.117
6.5
-
-
Zea mays