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Literature summary extracted from

  • Kamsteeg, J.; van Brederode, J.; van Nigtevecht, G.
    The pH-dependent substrate specificity of UDP-glucose: anthocyandin 3-rhamnosylglucoside, 5-O-glucosyltransferase in petals of Silene dioica: the formation of anthocyanidin 3,5-diglucosides (1980), Z. Pflanzenphysiol., 96, 87-93.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.116 Ca2+ 1 mM CaCl2 stimulates 5-O-glucosylation of cyanidin 3-rhamnosyl-glucoside 1.6fold, pH 7.4 Silene dioica
2.4.1.116 Co2+ 1 mM activates Silene dioica
2.4.1.116 Mg2+ 1 mM activates Silene dioica
2.4.1.116 Mn2+ 1 mM activates Silene dioica
2.4.1.116 additional information glucosylation of cyanidin 3-glucoside at pH 6.5 is not stimulated by divalent metal ions Silene dioica

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.116 EDTA 1 mM, pH 7.5, inhibits formation of cyanidin 3-rhamnosylglucoside-5-glucoside but has no effect on the formation of cyanidin 3,5-diglucoside at pH 6.5 Silene dioica
2.4.1.116 Hg2+
-
 Silene dioica
2.4.1.116 Zn2+
-
 Silene dioica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.116 0.5
-
 UDPglucose
-
 Silene dioica
2.4.1.116 0.59
-
 UDPglucose reaction with + cyanidin 3-rhamnosylglucoside at pH 6.5 Silene dioica
2.4.1.116 0.6
-
 UDP-glucose pH 6.5 and pH 7.4 Silene dioica
2.4.1.116 0.67
-
 UDPglucose reaction with cyanidin-3-glucoside at pH 6.5 Silene dioica
2.4.1.116 2 3.4 anthocyanidin-3-O-glucoside pH 6.5 Silene dioica
2.4.1.116 3.6
-
 cyanidin-3-rhamnosylglucoside
-
 Silene dioica
2.4.1.116 3.6
-
 anthocyanidin-3-O-rutinoside pH 7.4 Silene dioica
2.4.1.116 23.4
-
 cyanidin-3-glucoside
-
 Silene dioica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.116 CaCl2 1 mM, 1.6fold stimulation of glucosyl transfer to anthocyanidin-3-O-rutinoside at pH 7.4, but not of glucosyl transfer to anthocyanidin-3-O-glucoside at pH 6.5 Silene dioica

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.116 Silene dioica
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.116 petal
-
 Silene dioica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.116 UDP-alpha-D-glucose + anthocyanidin 3-O-beta-D-rutinoside at pH 7.4, substrate specificity is pH-dependent Silene dioica UDP + anthocyanidin 3-O-beta-D-rutinoside 5-O-beta-D-glucoside
-
 ?
2.4.1.116 UDP-glucose + anthocyanidin-3-O-glucoside at pH 6.5, but not at pH 7.4, substrate specificity is pH-dependent Silene dioica UDP + anthocyanidin-3,5-O-diglucoside
-
 ?
2.4.1.116 UDP-glucose + cyanidin-3-O-D-rhamnosyl-1,6-D-glucoside
-
 Silene dioica UDP + cyanidin-3-O-[D-rhamnosyl-1,6-D-glucoside]-5-O-D-glucoside
-
 r
2.4.1.116 UDP-glucose + cyanidin-3-O-glucoside
-
 Silene dioica UDP + cyanidin-3,5-diglucoside
-
 ?
2.4.1.116 UDPglucose + cyanidin 3-O-D-glucoside
-
 Silene dioica UDP + ? depending upon pH, the enzyme catalyzes the formation of either anthocyanidin 3-rhamnosylglucoside or 3,5-diglucoside. Maximal formation of anthocyanidin 3-rhamnosylglucoside-5-gluside at pH 7.4, maximal formation of anthocyanidin 3,5-diglucoside at pH 6.5. Formation of cyanidin 3-rhamnosylglucoside-5-glucoside proceeds 5-10fold faster than the cyanidin 3,5-diglucoside formation ?
2.4.1.116 UDPglucose + cyanidin 3-O-D-rhamnosylglucoside
-
 Silene dioica UDP + ? depending upon pH, the enzyme catalyzes the formation of either anthocyanidin 3-rhamnosylglucoside or 3,5-diglucoside. Maximal formation of anthocyanidin 3-rhamnosylglucoside-5-gluside at pH 7.4, maximal formation of anthocyanidin 3,5-diglucoside at pH 6.5 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.116 UDP-glucose:anthocyanidin 3-rhamnosylglucoside 5-O-glucosyltransferase
-
 Silene dioica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.116 6.5
-
 activity with cyanidin-3-glucoside Silene dioica
2.4.1.116 6.5
-
 glucosyl transfer to anthocyanidin-3-O-glucoside, substrate specificity is pH-dependent Silene dioica
2.4.1.116 7.4
-
 activity with cyanidin-3-rhamnosylglucoside Silene dioica
2.4.1.116 7.4
-
 glucosyl transfer to anthocyanidin 3-O-rutinoside, substrate specificity is pH-dependent Silene dioica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.116 6 7.3 pH 6.0: about 80% of maximal activity, pH 7.3: about 50% of half-maximal activity, reaction with cyanidin 3-glucoside Silene dioica
2.4.1.116 7 8.5 about half-maximal activity at pH 7 and 8.5, reaction with cyanidin-3-rhamnosylglucoside Silene dioica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.116 7.6
-
 30 min, stable below Silene dioica
2.4.1.116 8
-
 30 min, about 10% loss of activity Silene dioica
2.4.1.116 8.5
-
 30 min, about 40% loss of activity Silene dioica