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Literature summary extracted from

  • Mucha, J.; Svoboda, B.; Frohwein, U.; Strasser, R.; Mischinger, M.; Schwihla, H.; Altmann, F.; Hane, W.; Schachter, H.; Glossl, J.; Mach, L.
    Tissues of the clawed frog Xenopus laevis contain two closely related forms of UDP-GlcNAc:alpha3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I (2001), Glycobiology, 11, 769-778.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.101 cloning of isozymes A and B, 2 separate genes, DNA sequence determination and analysis Xenopus laevis
2.4.1.101 functional expression of isozymes A and B in Spodoptera frugiperda Sf21 cells as glutathione-S-transferase fusion proteins via baculovirus infection, isozyme B is folded less efficiently Xenopus laevis

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.101 T223A isozyme B, improvement of properties of isozyme B to the level of isozyme A Xenopus laevis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.101 1.6
-
 pyridylaminated Man3GlcNAc2 recombinant isozyme A Xenopus laevis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.101 Golgi apparatus medial enzyme Xenopus laevis 5794
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.101 Mn2+
-
 Xenopus laevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.101 additional information
-
 type II membrane proteins: short N-terminal cytoplasmic tail of 4 amino acid residues, transmembrane domain of 22 residues, stem region of 81 residue for isozyme A and 77 residues for isozyme B, amino acid sequences alignment Xenopus laevis
2.4.1.101 65000
-
 x * 65000, recombinant isozyme B, SDS-PAGE Xenopus laevis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.101 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide Xenopus laevis key enzyme of biosynthesis of complex and hybrid N-glycans UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.101 Spodoptera frugiperda
-
-
-
2.4.1.101 Xenopus laevis
-
 2 isozymes A and B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.101 recombinant glutathione S-transferase fusion isozymes and mutants from insect Sf21 cells Xenopus laevis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.101 heart isozyme B, low content of isozyme A Xenopus laevis
-
2.4.1.101 kidney isozyme B, low content of isozyme A Xenopus laevis
-
2.4.1.101 liver isozymes A and B Xenopus laevis
-
2.4.1.101 muscle isozymes A and B Xenopus laevis
-
2.4.1.101 ovary isozymes A and B Xenopus laevis
-
2.4.1.101 SF-21 cell
-
 Spodoptera frugiperda
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.101 0.6
-
 recombinant isozyme A Xenopus laevis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.101 UDP + pyridylaminated Man3GlcNAc2
-
 Xenopus laevis ?
-
 ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R no activity with pyridylaminated substrate Spodoptera frugiperda UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R recombinant isozyme A and B mutant T223A Xenopus laevis UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R pyridylamine Xenopus laevis UDP + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide key enzyme of biosynthesis of complex and hybrid N-glycans Xenopus laevis UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-Asn-peptide
-
 ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 Xenopus laevis UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.101 ? x * 65000, recombinant isozyme B, SDS-PAGE Xenopus laevis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.101 GnT-I
-
 Xenopus laevis
2.4.1.101 UDP-N-GlcNAc:alpha3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I
-
 Xenopus laevis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.101 37
-
 assay at Xenopus laevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.101 6.3
-
 assay at Xenopus laevis